摘要
目的 探讨溃疡性结肠炎 (UC)患者肠黏膜活检组织细胞因子mRNA的表达及其与NF κB活化的关系 ,以及抗炎药物 (柳氮磺吡啶和糖皮质激素 )对其的影响。方法 31例来自四川大学华西医院的UC患者 (符合 1993年太原会议制定的UC诊断标准 )被纳入本研究。其中 17例使用过药物 (柳氮磺吡啶或柳氮磺吡啶 +糖皮质激素 )治疗 ,14例未用过任何与UC治疗相关的药物 ,11例同期结肠癌患者 (取其癌旁正常组织 )作为对照。采用 :(1)凝胶电泳迁移率改变分析检测核因子 (NF) κBDNA结合活性 ;(2 )逆转录聚合酶链反应检测白细胞介素 (IL) 1βmRNA和IL 8mRNA的表达。 结果 (1)UC患者肠黏膜活检组织IL 1βmRNA和IL 8mRNA表达与对照组相比明显升高 (P <0 0 5 ) ,且与NF κBDNA结合活性呈显著正相关 (IL 1β :r=0 836 3,P <0 0 5 ;IL 8:r=0 6 0 2 4 ,P <0 0 5 )。 (2 )糖皮质激素和柳氮磺吡啶明显抑制NF κB的活性 ,降低IL 1βmRNA和IL 8mRNA的表达。结论 (1)NF κB是UC细胞因子释放的关键调控因素 ,在UC的发生和发展中起着十分重要的作用。 (2 )糖皮质激素和柳氮磺吡啶可能通过抑制NF κB的活性 ,减少细胞因子的表达而起到抗炎作用。
Objective To investigate the activation of nuclear factor κB (NF κB) and its relationship with expression of cytokine mRNA in intestinal mucosal biopsy specimens from patients with ulcerative colitis(UC). Methods 31 cases with UC were included in the study. 17 cases received sulfasalazine (SASP) or SASP and glucocorticoid treatment.14 cases did not receive any medication related with UC. Normal mucosa from 11 colon cancer cases served as control. Ten pieces of intestinal mucosal biopsy specimens were obtained from each patient. NF κB DNA binding activity was evaluated with electrophoretic mobility shift assay(EMSA). Expression of cytokine mRNA were studied with reversal tanscription polymerase chain reaction (RT PCR).Results (1)The expression of IL 1β mRNA and IL 8 mRNA was increased significantly in patients with UC, as compared with that in the control specimens ( P <0.05) and had a significant positive correlation with NF κB DNA binding activity ( r =0 8363, P <0.05; r =0.6024, P <0.05, respectively). (2) Glucocorticoids and SASP strongly inhibited NF κB activation and signficantly decreased the expression of IL 1β mRNA and IL 8 mRNA. Conclusions NF κB is a major and essential factor in regulating the expression of cytokine and plays a fundamental role in the pathogenesis of UC. SASP and glucocorticoids decrease cytokine expression via inhibition of NF κB activation.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2002年第4期252-255,共4页
Chinese Journal of Internal Medicine
基金
国家自然科学基金资助项目 (3 0 170 42 6)
教育部高等学校博士学科点专项科研基金资助项目 (2 0 0 0 5 4)
四川省卫生厅科研基金资助项目 (2 0 0 0 1)
