摘要
目的探讨甘草酸对转化生长因子β1(TGF β1)刺激大鼠肝星状细胞(HSC)胞内信号传导通路的作用。方法体外分离、培养大鼠肝HSC,甘草酸与TGFβ1刺激的HSC共同孵育。逆转录聚合酶链反应法检测1-1000 μmol/L甘草酸对TGF β1刺激的HSC中Smad2、3、7 mRNA的表达;Western印迹法检测1-1000μmol/L甘草酸对TGF β1刺激的HSC中Smad2、3、7蛋白及Ⅰ、Ⅲ型胶原蛋白的表达水平。结果TGFβ1促进HSC中Smad2、3、7 mRNA及蛋白的表达,同时促进Ⅰ、Ⅲ型胶原蛋白的表达。1-1000μmol/L甘草酸抑制Smad2、3、7 mRNA及蛋白的表达,并且抑制Ⅰ、Ⅲ型胶原蛋白的表达,均呈剂量依赖性。结论甘草酸可能通过干预大鼠HSC中TGFβ信号通路而减少胶原合成和发挥抗纤维化作用。
Objective To investigate the role of glycyrrhizin on TGF β 1 stimulated signaling transduction in rat hepatic stellate cells （HSCs）. Methods The mice HSCs were isolated and cultured with or without glycyrrhizin （ 1 μmol/L - 1 000 μmol/L） in vitro after TGFβ 1 stimulation. The mRNA level of Smad2, 3. 7 were measured with RT-PCR; protein expression level of Smad2, 3. 7 and collagen I , Ⅲ were analyzed with Western blot. Results TGF β 1 increased the mRNA level and protein expression of Smad2, 3, 7 in HSC; it also increased protein expression of collagen I and Ⅲ. 1 μ mol/L -1000 μ mol/L glycyrrhizin decreased the mRNA level and protein expression of Smad2,3,7; it also inhibited protein expression of collagen I and III gradually. Conclusion Interventing the TGF signaling pathway and decreasing the synthesis of collagen, might be involved in the anti-fibrosis mechanism of glycyrrhizin.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2005年第11期828-831,共4页
Chinese Journal of Hepatology
