摘要
目的:建立离子对色谱法测定阿替洛尔注射液的含量。方法:Betasil C_(18)(250 mm×4.6 mm,10μm)色谱柱,磷酸氢二钾溶液-甲醇-辛烷磺酸钠(800∶200∶0.5)为流动相,检测波长:275 nm,流速:1.0 ml·min^(-1)。结果:阿替洛尔在30～105μg·ml^(-1)浓度范围内,线性关系良好,r=0.999 8。平均回收率为99.8%(RSD:0.6%,n=9)。结论:HPLC法简便快速,稳定可靠可作为阿替洛尔注射液的质量控制方法。
Objective： To establish a method for determination the content of atenolol in atenolol injections by an ion-pair HPLC. Method： The Betasil C 18 column（250 mm × 4.6 mm, 10 μm）was used; the mobile phase consisted of phosphate buffer-methanol-so- dium octanesulfonate （800： 200：0.5 ） ;the detection wavelength was 275 nm;the flow rate was 1.0 ml. min -1. Result： The linear range of atenolol was 30 - 105 μg. ml - 1 （ r = 0.999 8 ）. The average recovery was 99.9% with RSD 0.6% （ n = 9 ）. Conclusion： The method is simple, rapid, stable and reliable,it can be used for the quality control method for atenolol injections.
出处
《中国药师》
CAS
2008年第9期1068-1069,共2页
China Pharmacist
关键词
离子对色谱法
阿替洛尔
含量测定
Ion-pair HPLC
Atenolol injections
Content determination
