摘要
目的建立RP-HPLC-FLD方法测定血浆中阿替洛尔的浓度。方法血浆样品用有机溶剂（乙酸乙酯-异丙醇=4：1）提取浓缩后测定，内标法定量。色谱柱为Phenomenex C18柱（4．6mm×250mm，5μm），流动相为乙腈-水（含25mmol·L^-1磷酸二氢钠和5mmol·L^-1十二烷基磺酸钠）=30：70（v／v）；激发波长：226nm，发射波长：310nm。结果阿替洛尔浓度在9．6～1200．0ng·mL^-1呈良好线性关系（r=0．9993），最低定量下限浓度为9．6ng·mL^-1，方法回收率为94．3％～104．5％（n=15），日内RSD为6．1％～11．8％（n=15），日间RSD为6．0％～11．1％（n=45）。结论本方法准确、简单、灵敏，可用于阿替洛尔的药动学研究。
Objective To establish an RP-HPLC FLD method for the determination of atenolol in human plasma. Methods Atenolol was extracted by organic solvent （acetoacetate-isopropanol=4 ： 1） and determined, quantified with internal standard. The separation was performed on Phenomenex C18 （4.6mm×250mm,5μm） with a mobile phase consisting of water （25 mmol·L^-1 sodium dihydrogen phosphate and 5 mmol·L^-1 sodium laurylsulfate） and acetonitrile （70 ： 30, v/v）. The detective wavelength was as follows： λex ： 226 nm, λem = 310 nrru Results The linear range was 9.6-1 200.0 ng·mL^-1 （r=0. 999 3）. The lower limit of quantification （LLOQ） was 9.6 ng·mL^-1. The method recoveries from plasma were within 94.3%-104.5% （n= 15）. Intra-day and inter-day RSD was within 6.1%11.8% （n=15） and 6.0%-11.1% （n=45）, respectively. Conclusion The method is accurate, simple and sensitive, and suitable for research on the pharmacokinetics of atenolol.
出处
《中南药学》
CAS
2009年第5期341-343,共3页
Central South Pharmacy
