摘要
籼-粳分化在亚洲栽培稻(Oryza sativa L.)的驯化过程中非常重要,但其分化机制仍不清楚.有的学者认为水稻籼-粳分化是亚洲栽培稻在驯化过程中适应不同生境的结果,也有人认为籼-粳分化在水稻的野生祖先种中就已经存在.为了研究普通野生稻的籼-粳分化,并解析稻属(Oryza)植物的籼-粳遗传变异,利用34对籼-粳特异插入/缺失分子标记(InDel)引物,研究了50份典型籼稻(O.sativaL.subsp.Indica Kato)和粳稻(O.sativaL.subsp.japonica Kato)样本以及来源于35个国家的348份稻属其他种材料.结果表明,亚洲栽培稻存在明显的籼-粳分化,普通野生稻复合体(O.rufipogoncomplex)中存在"偏籼"和"偏粳"类型,而稻属的其他种均未出现籼-粳分化,普通野生稻复合体中"偏籼"和"偏粳"类型的地理分布格局与籼稻和粳稻的地理分布格局相吻合.考虑到大部分普通野生稻复合体的样本取自邻近有栽培稻种植的普通野生稻群体,推测得出部分普通野生稻样本中表现出的"偏籼"和"偏粳"类型可能是栽培稻的籼稻品种和粳稻品种在普通野生稻的长期协同进化过程中基因交流的结果.
Indica-japonica differentiation is the most significant genetic variation among varietal types of Asian cultivated rice （ Oryza sativa L. ）. Despite its important roles in the domestication process of Asian cultivated rice, the mechanisms of indica-japonica differentiation are still unclear. One of the hypotheses stresses that the differentiation of indica-japonica has occurred in the wild ancestral species. In order to investigate the indica-japonica genetic variation in all wild relatives in the genus Oryza, 50 typical indica and iaponica rice cultivars and 348 wild Oryza accessions from 35 countries were included in the study using 34 indica-japonica specified InDel primer pairs. The results indicated that significant indicajaponica differentiation occurred in Asian cultivated rice, and some ＂indica＂ and ＂japonica＂ types were also found in some of the O. rufipogon complex. However, no such differentiation were detected in other Oryza species. The geographical distribution pattern of ＂indica＂ and ＂japonica＂ types in the O. rufipogon complex is associated with the geographical distribution of indica and japonica rice varieties. Given the wild accessions were collected in habitats adjacent to the fields of cultivated rice, we believe that the ＂indica＂ and ＂japonica＂ types in O. rufipogon complex is more likely generated by the introgression from the respective ＂indica＂ or ＂japonica＂ rice varieties historically.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2008年第3期281-287,共7页
Journal of Fudan University：Natural Science
基金
科技部“973”计划资助项目(2006CB100205)
关键词
稻属植物
特异插入/缺失分子标记
籼-粳分化
遗传变异
genus Oryza
specific InDel primer pairs
indica-japonica differentiation
genetic variation
