摘要
本研究利用36对InDel分子标记引物对贵州地方水稻种质的籼-粳遗传分化和亲缘关系进行分析,结果表明,82份贵州地方栽培稻中49份为粳稻,33份为籼稻,贵州地方栽培稻"禾"品种主要属于粳稻,而"谷"品种主要为籼稻。基于Nei氏遗传距离的亲缘关系分析表明在粳稻群体和籼稻群体中均存在与野生稻亲缘关系近的品种,其中的粳稻品种与野生稻的遗传关系比之籼稻品种近。而基于MCMC算法的遗传结构分析揭示了贵州地方籼稻品种中存在较为复杂的遗传结构。分子变异分析显示,粳稻和籼稻品种的遗传变异主要来自亚种内,遗传多样性分析表明其亚种内籼稻品种的遗传多样性略高于粳稻品种。研究结果揭示了贵州省黔东南地区栽培稻种质资源的籼-粳分化程度、遗传关系及其遗传多样性。
36 InDel primer pairs were used to analyze the Indica-]aponica differentiation and genetic relationship of 82 rice varieties in Guizhou province.The results showed that 82 rice varieties were divided into 49japonica and 33 indica, most of the landraces ＂He＂ were classified as japonica, while ＂Gu＂ were mainly classified as indica. Analysis of genetic relationship based on Nei＇s genetic distance among those varieties showed that several landraces have a close relationship with Oryza rufupogon, and the japonica varieties have a closer relationship with rufupogon when compared with indica varieties. The population structure detected by Structure 2.3 and revealed a more com- plex structure in indica landraces and japonica landraces. The result of AMOVA analysis suggested that the genetic variation mainly existed within subspecies, and the genetic diversity in indica varieties is higher than japonica vari- eties according to Nei＇s genetic diversity and Shannon index. The results revealed the Indica-Japonica differentia- tion, relationship and genetic structure among Guizhou rice landraces.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2013年第4期486-496,共11页
Genomics and Applied Biology
基金
国家科技支撑计划"喀斯特山区水稻
油菜等作物安全转基因技术应用及种质创新"(2007BAD59B06)
贵州省重大科技专项"贵州特有水稻种质资源优异基因克隆及育种技术研究应用"(黔科合重大专项字[2012]6005号)共同资助
关键词
水稻
籼-粳分化
插入/缺失
遗传结构
Rice, lndica-Japonica differentiation, Insertion/deletion, Genetic structure ＇
