摘要
采用40对SSILP、30对InDel和43对SSR标记对来自斯里兰卡的28份杂草稻和14栽培稻,来自国内外的9份籼型栽培稻、7份粳型栽培稻和4份代表性杂草稻进行遗传多样性分析。SSILP、InDel和SSR标记分别检测到84、61和213个等位基因,其平均多态性信息含量(PIC)分别为0.257、0.255和0.543。聚类分析显示供试的42份斯里兰卡杂草稻和栽培稻均为籼型。SSILP与InDel标记的相关性很高(r=0.989),而它们与SSR标记相关性较低(0.812和0.808)。结果表明,SSILP和InDel标记可高效鉴别各类稻种资源的籼粳属性,而SSR标记更适用于亚种内的分类。
Forty SSILP, 30 InDel and 43 SSR markers were used to compare genetic diversity of 42 weedy and cultivated rice entries from Sri Lanka, 9 indica and 7 japonica rice cultivars as well as four representatives of weedy rice from China and other countries（regions）. There were 84, 61 and 213 alleles identified by SSILP, InDel and SSR markers, with average polymorphism information content （PIC） values 0. 257, 0. 255 and 0. 543, respectively. The N-J clusters analysis indicated all 42 Sri Lanka weedy and cultivated rice entries belonged to indica type. The genetic distances of SSILP and InDel markers revealed highly correlation （r=0. 989）. Meanwhile the associations between SSR markers and the other two markers （SSILP and InDel） were lower as 0. 812 and 0. 808, respectively. With the rising reliability and accuracy of indica japonica classification resulted from the development of more subspecies-specific markers, SSILP and InDel markers can be applied extensively for the indica-japonica classification of rice germplasm. And SSR markers can classify better in the subspecies.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2012年第6期686-692,共7页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目(30860057)
国家973计划资助项目(2011CB100401)
关键词
杂草稻
栽培稻
分子标记
籼粳分类
weedy rice
cultivated rice
molecular marker
indica japonica classification
