摘要
目的:探讨菟丝子总黄酮对大鼠流产模型蜕膜PR及Th1/Th2细胞因子表达的影响,同时观察血清P和PR的变化。方法:对溴隐亭致SD孕鼠流产模型,设菟丝子总黄酮高、低剂量治疗组、达芙通为阳性对照组,于孕12d处死各组大鼠,酶联免疫检测血清P和PRL的水平,免疫组化法检测蜕膜PR的表达,RT-PCR法检测蜕膜Th1/Th2 mRNA的表达。结果:模型组蜕膜IL-4、IL-10 mRNA及PR蛋白表达明显低于正常妊娠组,TNF-α、IFN-γmRNA表达明显高于正常妊娠组,差异有显著性(P<0.05),菟丝子总黄酮可上调流产大鼠蜕膜IL-4、IL-10、PR的表达,下调TNF-α、IFN-γ表达,同时可提高血清P的水平,其作用与孕激素达芙通相似。结论:菟丝子总黄酮可能通过调节母胎界面内分泌-免疫网络平衡而起到维持早孕的作用。
Objective： To study the effect of TFCC on expression of Th type-I/Th type-2 cytokines and PR in rats abortion model, and observe the changes of serum P and PRL. Methods： Early abortion rat model was established by Bromocriptine at 6-8 gestation day, the model rats were treated with high and low dosage of TFCC and the progesterone as the positive control. Progesterone and prolaetin levels in the serum were measured by radioimmunoassay, the protein expression of PR was inspected by immunohistoehemieal, RT-PCR was applied to determine the expression of Tbl/Th2 mRNA on deciduas. Results ： In the deciduas of model group, IL4 and IL-10 cytokine contens were clearly lower than that of normal pregnancy group. The difference was notable（ P 〈 0.05 ） , In the deciduas of model group, IFN-γ,/and TNF-α cytokine contents were clearly higher than that of normal pregnancy group. The difference was nota- ble（P 〈0. 05）, TFCC could up-regulate the level of IL4 and IL-10, and down-regnlate the level of TNF-α and IFN-γ/,which could promote P secretion and up regulate PR mRNA expression on deciduas. Conclusion： TFCC can regulate endocrinologycal and immunological network equilibration on maternal-fetal face, finally achieve prevention abortion.
出处
《中药材》
CAS
CSCD
北大核心
2008年第8期1201-1204,共4页
Journal of Chinese Medicinal Materials
基金
广东省自然科学基金(5300997)
广州市卫生局资助项目(2005A041)
关键词
自然流产
菟丝子总黄酮
TH1/TH2细胞因子
蜕膜孕激素
Spontaneous abortion
Total flavones from Cuscuta chinensis （TFCC）
Th type-1/Th type-2 cytokines
PR
