摘要
目的采用网络药理学的方法探讨菟丝子治疗骨质疏松症可能的作用机制。方法通过检索TCMSP、TCMID、SymMap、BATMAN-TCM、ETCM以及查阅文献获取菟丝子的全部成分,并筛选菟丝子活性成分以及治疗骨质疏松症的靶标;采用Cytoscape 3.6.0构建菟丝子的"活性成分-靶标"网络并结合数据库String和Cytoscape 3.6.0绘制所得靶标间相互作用关系;最后利用DAVID数据库对靶标基因进行GO功能分析与KEGG富集分析。结果筛选出的与骨质疏松症相关的活性成分有14个,包括槲皮素、芹菜素,金丝桃苷等;活性成分发挥作用的潜在靶标有83个,主要有白细胞介素-6(IL-6)、血管内皮生长因子A(VEGFA)、骨分化特异性转录分子2(RUNX2)、雌激素受体α(ESR1)等;KEGG富集分析得到与骨质疏松症紧密相关通路主要有PI3K/Akt、MAPK、TNF信号通路等。结论中药菟丝子中槲皮素、芹菜素,金丝桃苷等活性成分可能通过PI3K/Akt、MAPK、TNF等信号通路实现对骨质疏松症的防治。
Objective To study the mechanism about Cuscuta Chinensis in the treatment of osteoporosis by network pharmacology.Methods The total components of Cuscuta Chinensis were obtained by searching TCMSP,TCMID,Symmap,BATMAN-TCM,ETCM and literature.Screening the targets of Cuscuta Chinensis in the treatment of osteoporosis;Cytoscape 3.6.0 is used to construct the"active component-target"network of Cuscuta Chinensis;The DAVID database was used to perform GO function analysis and KEGG enrichment analysis.Results TCMSP,TCMID,SymMap,BATMAN-TCM,ETCM and literature were searched to obtain all components of Cuscuta Chinensis,and 14 active ingredients related to osteoporosis were screened,including:quercetin,apigenin,and hyperin,etc;There are 83 potential targets for active ingredients including:IL-6,VEGFA,RUNX2,ESR1,etc;KEGG enrichment analysis shows that the pathways related to osteoporosis includeing PI3 K/Akt,MAPK,and TNF signaling pathways.Conclusion Quercetin,apigenin,hyperin and other active ingredients in Cuscuta Chinensis may prevent and treat osteoporosis through signal pathways of PI3 K/Akt,MAPK,and TNF.
作者
刘博男
宋辉
薛屹
孙向明
胡扬
李文兰
LIU Bonan;SONG Hui;XUE Yi;SUN Xiangming;HU Yang;LI Wenlan(School of Pharmacy,Harbin University of Commerce,Harbin 150076,China;Drug Engineering Technology Research Center,Harbin University of Commerce,Harbin 150076,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2020年第7期2399-2406,共8页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
哈尔滨商业大学2019年研究生创新科研基金项目(YJSCX2019-607HSD):菟丝子黄酮单体成分配伍的拟雌激素活性协同作用研究,负责人:李文兰
黑龙江省自然科学基金重点项目(ZD2017014):基于体内过程和协同效应的菟丝子拟雌激素作用物质研究,负责人:李文兰
哈尔滨商业大学校级科研项目(18XN083):肉苁蓉总苷体内代谢过程与拟雌激素作用关联性研究,负责人:李文兰
关键词
菟丝子
骨质疏松症
网络药理学
分子机制
Cuscuta chinensis
Osteoporosis
Network pharmacology
Molecular mechanisms
