摘要
目的:研究地龙降压蛋白对自发性高血压(SHR)模型大鼠的降压作用及对血管紧张素Ⅱ(AngⅡ)、AT1受体表达的影响。方法:单次静脉注射地龙降压蛋白,观察SHR模型大鼠血压的动态变化;地龙降压蛋白连续干预28d,观察对SHR模型大鼠血压变化;同时检测大鼠AngⅡ含量及AT1受体的表达。结果:单次及多次给药,地龙降压蛋白均可显著降低SHR模型大鼠血压(P<0.05);地龙降压蛋白能显著降低SHR模型大鼠血浆AngⅡ水平(P<0.05),而肾脏局部AngⅡ含量虽有所下降,但无显著性差异(P>0.05);与正常对照组比较,SHR模型大鼠肾脏局部AT1受体表达显著增加,经地龙降压蛋白干预,其表达减少。结论:地龙降压蛋白对SHR有降压作用,其降压机制可能与降低AngⅡ含量及抑制肾脏AT受体表达有关。
OBJECTIVE： To investigate the effects of the antihypertensive protein from Pheretima on blood pressure, angiotensin Ⅱ and angiotensin Ⅱ AT1 receptor in spontaneously hypertensive rats （SHR） .METHODS： The dynamic change of blood pressure in SHR after singel intravenous injection of antihypertensive protein from Pheretima was observed. After intervention with antihypertensive protein from earthworm for 28 d,the levels of blood pressure, angiotensin Ⅱ and expression of angiotensin Ⅱ AT1 receptor in SHR were detected. RESULTS： Either single intravenous injecion or multiple dosing of the antihypertensive protein from Pheretima could significantly reduce the blood pressure in SHR （P 〈 0.05） ; the antihypertensive protein from Pheretima could markedly lower the angiotensin Ⅱ level in plasma （P〈0.05） and slightly lower the angiotensin Ⅱ level in tissue of kidney but the difference was nonsignificant （P 〉0.05） .The expression of angiotensin Ⅱ AT1 receptor in kidney of SHR model increased significanlty compared the normal control （wistar rats）, but decreased after the intervention of the antihypertensive protein from Pheretima.CONCLUSION： The antihypertensive protein from Pheretima has antihypertensive effect on SHR. The mechanism may be related to the reduction of angiotensin Ⅱ level and lowering of the expression of a angiotensin Ⅱ AT1 receptor in kidney.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第24期1850-1852,共3页
China Pharmacy
关键词
地龙降压蛋白
自发性高血压
血管紧张素Ⅱ
AT1受体
Antihypertensive protein from Pheretima
Spontaneously hypertensive rat（SHR）
AngiotensinⅡ
An-giotensin Ⅱ AT1 receptor
