摘要
目的:探讨基质金属蛋白酶9(MMP-9)和金属蛋白酶组织抑制剂1(TIMP-1)在高氧致慢性肺疾病(CLD)新生大鼠肺组织中的动态变化和意义。方法:足月新生大鼠生后12h分别持续吸入0.90～0.95的高氧和空气,于1,3,7,14,21d应用免疫组化和RT-PCR方法分别检测肺组织MMP-9和TIMP-1蛋白及mRNA表达。结果:MMP-9在高氧3d时蛋白和mRNA表达均较空气组增强(P<0.05,P<0.01);TIMP-1蛋白在高氧组表达高于空气组,3d和7d比较P<0.05,14d和21d比较P<0.01;TIMP-1 mRNA水平高氧组高于空气组,3d和7d比较P<0.05,和14d比较P<0.01,和21d比较无差异。结论:高氧暴露后MMP-9和TIMP-1的表达在不同阶段的变化不一致,MMP-9/TIMP-1平衡状态遭到破坏,可能是高氧后胶原异常沉积以及正常肺泡化过程受阻的重要因素。
Objective： To explore the dynamic expressions of matrix metalloproteinase-9 （MMP-9） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in the lung tissue of neonatal rats with hyperoxia-induced chronic lung disease （CLD）. Methods： Full-term newborn rats were continuously exposed to oxygen （0.90-0.95） or room air within 12 hours after birth. The changes in MMP-9 and TIMP-1 protein and mRNA expressions were measured by immunohistochemical method and reverse transcription polymerase chain reaction on days 1,3,7,14,and 21. Results： The expressions of MMP-9 protein and mRNA on day 3 were significandy higher in hyperoxia group than in room air group （P 〈 0.05,P 〈 0.01）,but no between-group difference was found at other time points. The expression of TIMP-1 protein on days 3 and 7 was higher in hyperoxia group than in room air group,and on days 14 and 21 it was significantly higher in hyperoxia group than in room air group. The expression of TIMP-1 mRNA on days 3 and 7 was higher in hyperoxia group than in room air group, and on days 14 it was significantly higher in hyperoxia group than in room air group. No between-group difference in the expression of TIMP-1 mRNA was found on day 21. Conclusion： With the prolongation of hyperoxia, the expressions of MMP-9 and TIMP-1 change inconsistently. The balance between MMP-9 and TIMP-1 is disturbed,which may play an important role in the collagen deposition in lung interstitium and the inhibition of alveolar development in CLD.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2007年第5期502-504,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(30440056)
