摘要
研究了利用无机载体（活性炭、氧化铝、分子筛等）吸附法和天然藻胶（海藻酸钙等）包埋法制备的固定化甲烷氧化细菌的催化性能及其在生物反应器中的反应．结果表明，在进行甲烷制甲醇的反应中，活性炭吸附制备的固定化细胞的操作稳定性最好，但其初始酶活性与休止游离细胞相比损失了６０％～８０％；海藻酸钙包埋的固定化细胞初始酶活性高（与游离细胞相比，可保持５５％～９０％的酶活性），但反应中甲醇累积速率很低；而双重介质（琼脂陶瓷粒）包埋的固定化细胞有利于保持固定化细胞的酶活性，增加操作稳定性。
Methanotrophic bacterial cells were adsorbed on inorganic supports (activated carbon, alumina, zeolite etc.)or entrapped in gels (Caalginate or agar). The biocatalytic properties of these biocatalysts were studied in a liquidsolid bioreactor. The operational stability of microbial cells immobilized on activated carbon was the best, but its initial enzymatic activity decreased by 60%～80% as compared with resting free cells. Immobilized cells entrapped in Caalginate gel had higher retention (80%～90%) of cells enzymatic activity, but the operational stability decreased quickly, because Ca2+ stimulated the activity of methanol dehydrogenase resulting in methanol oxidation. Immobilization using twin inorganic supports (supports and gel) is a simple, universal and available method. It seems a better biocatalyst for high activity retention and enhanced stability in the methane oxidation reaction.
出处
《催化学报》
SCIE
CAS
CSCD
北大核心
1997年第6期503-507,共5页
基金
中国科学院"八五"重点应用项目
关键词
固定化细胞
甲烷单加氧酶
甲烷
甲醇
生物催化
Immobilized cells, Methanotrophic bacteria, Methane monooxygenase, Methane, Methanol, Biocatalysis
