摘要
聚-3-羟基丁酸酯(Poly-3-hydroxybutyrate,PHB)作为一种新的塑料替代品,具有良好的生物相容性和生物可降解性,目前已成为食品包装领域研究的热点。甲基弯菌(Methylosinus trichosporium)IMV3011可以利用单碳碳源在非灭菌条件下胞内合成PHB。为获得能够利用低成本碳源高产PHB的菌种,对甲基弯菌IMV3011积累PHB的能力进行了研究。对碳源的种类、供给形式、供给量和培养基组分的研究结果表明,甲基弯菌对甲醇的耐受能力提高后,加入0.1%甲醇可使甲烷中的该类菌种达到较高的生长密度(2.5g/L);通过对第1阶段培养期的选择和第2阶段培养基组分(NH4+、NO3-、H2PO4-、Mg2+、Cu2+、Fe3+)的调整,有效缓解了第2阶段的优化培养基中细胞生长与PHB积累的矛盾,实现了两个阶段细胞量的增长及第2阶段单位细胞PHB积累量的增长,提高了PHB的含量(34.9%)与产量(0.6g/L);通过对各种条件的研究,发现相对分子质量的可调控性,PHB相对分子质量在106以上。
Poly-3-hydroxybutyrate （PHB） has already become the hot spot research of food packing area because of having excellent biological compatibility and degeneration as a kind of new plastic substitute. The methanotrophic strain Methylosinus trichosporium IMV 3011 is able to synthesize PHB with methane in a brief non-sterile process. In order to obtain microbial species of high production PHB low carbon cost, the ability of accumulating PHB was studied. Through inspecting the type of carbon, the supply form, the supply quantity and the culture medium component, the results indicated that： when 0.1% liquid methanol added into 100mL culture medium under a methane/air gas mixture （1：1, V/ V）, the cell concentation could reach 2.5 g/L. Cultivation was performed in two stages： a continuous growth phase and a PHB accumulation phase under different concentration of essential nutrient （ammonium, nitrate, phosphorus, magnesium iron or copper） in batch culture. Then the suitable time in first stage and cultivation condition in second stage to produce PHB have been obtained. The PHB content of biomass was as high as 34.9% （0.6 g/L） . It is also found the molecular mass of PHB produced high up to 10^6 could be controlled by some conditions.
出处
《中国食品学报》
EI
CAS
CSCD
2008年第3期5-11,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
教育部新世纪优秀人才支持计划资助(No.NCET-05-0356)
