摘要
对彩色马蹄莲进行组培快繁研究,结果表明:丛芽增殖的最佳因素组合为MS+6-BA 0.5 mg/L+TDZ 2 mg/L;在诱导愈伤组织出芽时,先用适当浓度的6-BA来诱导愈伤组织产生不定芽,再将其转接到低浓度的NAA上以促进不定芽成苗。MS+NAA0.5 mg/L+活性炭5%有利于生根。
Cell culture was made of Zaritedeschia antedschia for its rapid propagation. Orthogonal design indicated that MS ＋ 6-BA0.5 mg/L ＋ TDZ 2mg/L was the best culture medium to induce proliferation buds from its tuber. The prime process of inducing buds from the callus was to use the 6-BA at the first step and then treat the buds with low concentration of NAA. The best culture medium for multiplication of cluster shoots was MS ＋ NAA 0.5 mg/L ＋ AC 5 %.
出处
《西南农业学报》
CSCD
2008年第3期775-778,共4页
Southwest China Journal of Agricultural Sciences
基金
四川省应用基础研究项目(03JY029-096-2)
关键词
彩色马蹄莲
组培研究
快速繁殖
Zantedeschia antedschia
tissue culture
rapid propagation
