摘要
为了提高大丽轮枝菌(Verticillium dahilaeKleb.)拮抗细菌枯草芽孢杆菌Bacillus sub-tilisLZ2-70菌株的芽孢形成率及芽孢数量,在摇瓶发酵基础上对影响该菌芽孢形成的主要因素进行了考察。通过单因素试验确定了最佳的碳源、氮源和无机盐等,通过正交试验研究了培养基最佳浓度和组合,以及种龄、接种量、培养液初始pH和培养时间等发酵条件。摇瓶发酵生产芽孢的最佳条件为:0.5%麦芽糖、2%黄豆饼粉、0.05%KH2PO4和0.1%MnSO4.H2O、培养液初始pH值8.0、种龄24 h、装瓶量30 mL/250 mL、接种量10%、发酵时间48 h、培养温度为30℃、摇床转速为200 r.min-1。在此优化条件下,发酵液中LZ2-70菌株的芽孢数量达到6.6×109个.mL-1,芽孢形成率为98.94%。
In order to improve the spore production rate and spore quantity of antagonistic strain Bacillus subtilis LZ2-70 against Verticillium dahIiae, the main factors influencing spore production were investigated using shake-flask fermentation method. Through single factor experiment and orthogonal experiment, the optimal shaking flask fermentation condition of strain Bacillus subtilis LZ2-70 was determined as follows： media composed of 0.5% Maltose, 2% soybean meal, 0.05% KH2PO4 and 0.1% MnSO4 · H2O, initial pH 8.0, culture time 24 h and bottle filling capacity 30 mL/250 mL, 10% inoculums volume, fermentation time 36 h, fermentation temperature 30℃, rotating speed 200 r · min^-1. Under this optimum condition, the spore quantity of strain Bacillus subtilis LZ2-70 achieves 6.6×10^9· mL^-1 and the spore production rate achieves 98.94%.
出处
《棉花学报》
CSCD
北大核心
2009年第4期307-312,共6页
Cotton Science
基金
河北省自然科学基金项目
关键词
大丽轮枝菌
拮抗细菌
枯草芽孢杆菌
发酵
芽孢
Verticillium dahilae
antagonistic bacteria
Bacillus subtilis
fermentation
spore
