摘要
目的研究骨关节炎（OA）患者关节软骨细胞凋亡和软骨破坏的关系，为用药物控制凋亡作为治疗OA的潜在治疗策略提供理论依据。方法应用原位凋亡方法（TUNEL）检测OA患者（n=20）以及以股骨颈骨折患者为对照组（n=8）的关节软骨细胞的凋亡情况，并通过凋亡软骨细胞的分布来半定量地评估软骨的破坏程度；应用免疫组织化学方法检测凋亡相关蛋白survivin（SVV）、caspase．3和bcl-xl的表达，研究OA软骨破坏与凋亡蛋白表达的相关性。结果在软骨破坏的相对早期即可以检测凋亡软骨细胞，而且与软骨破坏的程度显著相关（r=0．476，P=0．007），正常对照组组织则含有较少凋亡细胞（P=0．000）。SVV表达在有降解损害的软骨细胞和软骨表层细胞中，与正常对照组之间差异有统计学意义（r=0．413，P=0．008），而且与TUNEL染色阳性凋亡细胞的数量有一定的相关性。与SVV相比，caspase-3表达范围较广，在相对正常的软骨区域也可检测到。软骨组织中未见明确的bcl-xl蛋白表达。结论OA软骨细胞凋亡的程度与软骨破坏程度密切相关，在软骨的降解损害过程中，SVV和caspase-3蛋白的表达对OA软骨细胞的凋亡过程起到一定的作用。
Objective To investigate the relationship of chondrocyte apoptosis to cartilage destruction in osteoarthritis （OA）. Methods Specimens of cartilage were collected from 20 OA patients and 8 patients with femoral neck fracture during operation. TUNEL method was performed to semi-quantitatively detect the distribution of apoptotic chondrocytes. Immunohistochemistry was used to detect the expression of the apoptosis-associated proteins ： surviving （ SVV ） , caspase-3, and apoptosis regulating protein Bcl-2. Results Apoptotic chondrocytes were identified in a relatively early phase of cartilage destruction, and correlated positively and significantly with the degree of cartilage degeneration. In contrast, the specimens of normal subjects contained few cells with apoptotic changes （ P = 0. 000 〈 0.05 ）. The TUNRL positive cell rate of the OA group was significantly higher than that of the control group （P = 0. 000）. Protein expression of SVV was observed in the chondrocytes within the degraded lesions and in the chondrocytes of the superficial layers, and showed a kind of positive correlation with the number of TUNEL-stained cells. The distribution area of caspase-3 was greater than that of SVV, and could be detected in relatively normal cartilage areas. Protein expression of Bcl-xl was not definitely detected in cartilage tissue. Conclusion The degree of chondrocyte apoptosis is closely related to cartilage destruction. The protein expression of SVV and caspase-3 may reflect the involvement of these proteins in the apoptotic process in the articular chondrocytes in OA.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2008年第19期1339-1341,共3页
National Medical Journal of China
关键词
骨关节炎
软骨细胞
凋亡
Osteoarthritis
Chondrocyte
Apoptosis
