摘要
目的了解2007年在辽宁省分离的乙型脑炎病毒基因型别及其病毒E基因分子特征。方法2006年8月在辽宁省东港市采集蚊虫标本，利用组织培养细胞进行病毒分离，对病毒分离物进行血清学和分子生物学鉴定。结果从采集的30批，共1500只三带喙库蚊标本中分离到2株病毒，命名为LNDG07-02、LNDG07-16，经鉴定均为基因1型乙脑病毒。病毒E基因区段核苷酸和氨基酸序列与乙脑减毒活疫苗株（SA14-14-2株）的同源性分别为87．8％-88．0％和97．2％，新分离病毒E基因区段与疫苗株存在11处氨基酸位点差异，与2002年在辽宁省分离的乙脑病毒相比，未发现氨基酸位点变异。结论自2002年以来在东港市再次分离到基因1型乙脑病毒，与2002年在辽宁分离的基因1型乙脑病毒相比E基因区段氨基酸未发生变异。基因1型乙脑病毒在辽宁省东港市持续存在。
Objective To isolate Japanese Encephalitis virus （JEV） from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene. Methods Collected mosquitoes in Dandong Liaoning province in August, 2006. Virus isolation was using issue culture cells. Isolated viruses were identified by using serological and molecular methods. Results Two new JEV strains, LNDG07-02, LNDG07-16, were isolated from 1500 mosquitoes were belonging to genotype 1. The identity of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaceine strain SA14-14-2 were 87.8%-88.0% and 97.2% , respectively. Total 11 amino acid sites were differences in E gene between new isolates and SA14-14-2. However, there were no differentiation between the new JEV strains and the isolates in Donggang 2002. Conclusion C, enotype 1 JEV was isolated again from Donggang, since the first isolation of this genotype in 2002. Genotype 1 JEV continues in existence in Donggang Liaoning province.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2008年第2期91-94,共4页
Chinese Journal of Experimental and Clinical Virology
基金
基金项目：中日传染病项目资助（Japanese Science and Health Foundation）
关键词
脑炎病毒
日本
基因型
种系发生
Encephalitis virus, Japanese
Genotype
Phylogeny
