摘要
目的对广西流行性乙型脑炎(乙脑)流行区的蚊虫进行乙脑病毒分离鉴定。方法2005年6、7月在北流市和靖西县居民区的畜圈内采集蚊虫标本,用C6/36和BHK21细胞分离乙脑病毒,用ClustalX(1.8)、Mega3.1等软件进行分离株E基因序列比对和系统进化分析。结果两地共采集三带喙库蚊771只、骚扰阿蚊425只,从三带喙库蚊中分离到3株基因1型乙脑病毒,3株病毒之间E基因片段核苷酸和氨基酸的最大同源性为99.3%和99.8%,与越南毒株之间核苷酸和氨基酸的最大同源性为98.4%和99.6%。结论从2005年采集自广西的蚊虫中分离到3株基因1型乙脑病毒,这是首次报道在广西分离到基因1型乙脑病毒。
Objective To isolate and identify Japanese encephalitis virus from mosquitoes collected in Guangxi,2005.Methods Mosquito samples were collected in Beiliu and Jingxi,Guangxi autonomous region.Japanese encephalitis virus was isolated by incubating C6/36 and BHK21 cells with mosquito homogenizations.RT-PCR was used to identify the isolates.E genes of the virus were sequenced and genotype of the virus was analyzed by bioinformatics software.ResultsA total of 1,196 mosquitoes（771 Culex tritaeniorhynchus and 425 Armigeres subalbatus） were collected and processed for virus isolation.3 strains of genotype 1 Japanese encephalitis virus were obtained from Culex tritaeniorhynchus.E gene nucleic acid and amino acid homology of the 3 isolates was up to 99.3% and 99.8% respectively.The homology of 3 isolates and E gene nucleic acid and amino acid of Vietnam Japanese encephalitis strains was up to 98.4% and 99.6% respectively.Conclusion 3 strains of Japanese encephalitis virus were isolated from mosquitoes collected in Guangxi.This was the first report of isolation of genotype 1 Japanese encephalitis virus in Guangxi.
出处
《疾病监测》
CAS
2010年第2期120-122,126,共4页
Disease Surveillance
基金
国家科技部重大传染病专项(No.2003BA12A08-01)
广西省卫生厅科研课题(No.Z2008204)~~
关键词
乙脑病毒
分离
鉴定
广西
Japanese encephalitis virus
isolation
identification
Guangxi
