摘要
目的 :测定癫痫患儿血清中丙戊酸的浓度。方法 :采用柱前衍生化法 ,用对溴苯甲酰甲基溴于 70℃反应 ,以壬酸为内标 ,乙腈 KH2 PO4 (0 0 5mol·L-1,pH7 0 )为流动相 ,经YWGODS柱 (2 0 0mm× 4 0mm)分离 ,2 5 4nm波长处二极管阵列检测器检测 ;16例服药的癫痫患者 ,设定时间抽血 ,测定血药浓度。结果 :在 8～ 2 0 0 μg·ml-1浓度范围 ,峰高比与浓度相关性良好 (r=0 9970 ) ,最低检测浓度为 4μg·ml-1,平均回收率为 97 2 4%± 3 86 % ,日内和日间RSD分别为 2 38% (n =9)和 4 18% (n =5 )。 16例癫痫患儿血药浓度监测结果平均值为 71 89± 2 4 5 4μg·ml-1。结论 :该方法快速、准确 ,血清用量少 。
OBJECTIVE:To monitor serum concentrations of valproic acid (VPA) in the treating of epileptic children.METHODS:VPA concentrations in serum were assayed by HPLC with YWG-ODS as column after derived using 4 bromophenacyl bromide which pelargonic acid was used as internal standard,and detected at 254 nm with the diodarray detector.RESULTS:The ratio of chromatiographic peak heights of VPA and internal standard correlated linearly with concentration of VPA rang 8～200 μg·ml -1 ( r =0.9997)and the average recovery was 97.74%± 3.86 % in the methodology.The minimum concentration of detection was 4 μg·ml -1 .The method is applied in the therapeutic drug monitoring of 16 children suffer from epileptic and the mean concentrations were 71.89±24.54 μg·ml -1 .CONCLUSION:The method is accurate,simple,fast,and can be used as routine test in the use of clinical individualize medication.
出处
《华西药学杂志》
CAS
CSCD
2001年第6期434-435,437,共3页
West China Journal of Pharmaceutical Sciences
