摘要
目的研究二氯二苯二氯乙烯(DDE)的类雌激素活性对人乳腺癌细胞株MCF-7增殖的影响。方法采用了体外MCF-7细胞增殖试验检测了DDE(3×10-5、3×10-6、3×10-7、3×10-8、3×10-9 mol/L)的类雌激素活性,并用生长曲线分析对其作用机制进行了初步探讨。结果3×10-6 mol/LDDE染毒组吸光度值高于溶剂对照组且MST-7细胞增殖的生长曲线与10-9 mol/L17β-雌二醇染毒组相似。结论DDE具有刺激MCF-7细胞增殖的类雌激素活性,其机制可能与17β-雌二醇相同。
Objective To evaluate the estrogen activity of DDE by using MCF-7 cell proliferation bioassay. Methods The estrogen activity of DDE was tested by using the MCF-7 cell multiplication experiment in vitro and the mechanism was explored preliminarily through growth curve analysis. Results DDE showed an obvious estrogenic activity in the MCF-7 cell proliferation and the cell cycle analysis indicated that cell proliferation index in DDE group was significantly higher than that in the control group. Conchlsion DDE has the estrogenic activity and the mechanism may be the same as that of E2r that is, binding with the estrogen receptor is the first step of the following biological effects.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2008年第3期236-237,共2页
Journal of Environment and Health
基金
中国疾病预防控制中心环境与健康相关产品安全所基金资助项目
关键词
农药
环境雌激素
细胞增殖
Pesticides
Environment estrogen
Cell multiplication
