摘要
目的:在体外建立子宫内膜腺上皮细胞原代分离和培养的方法,以获得高纯度子宫内膜腺上皮细胞。方法:选择正常子宫内膜组织,通过消化、过滤、选择性贴壁和二次消化等技术进行体外培养。结果:采用胶原酶二次消化法和选择性贴壁法成功实现子宫内膜腺上皮细胞的高纯度分离和原代培养。结论:采用胶原酶二次消化法和选择性贴壁法可高纯度分离子宫内膜腺上皮细胞及间质细胞。
Objective： To develop an improved method for the primary isolation and culture of highly purified endometrial glandular epithelial cells in vitro. Methods： Normal endometrial tissues were isolated and cultured in vitro by double collagenase digestion and selective wall-pasting. Results： Highly purified endometrial epithelial cells were successfully isolated and cultured. Conclusion： Epithelial and interstitial cells of the endometrium can be successfully isolated with high purity by double collagenase digestion and selective wall-pasting.
出处
《医学研究生学报》
CAS
2008年第5期472-474,I0003,共4页
Journal of Medical Postgraduates
基金
北京大学医学部"985工程"二期建设项目基金资助(批准号:985-2-015-24)
关键词
子宫内膜
细胞培养
腺上皮细胞
Endometrium
Cell culture
Glandular epithelial cell
