摘要
目的:建立一种简捷的分离纯化培养子宫内膜腺上皮细胞和基质细胞的方法。方法:选择子宫全切育龄妇女的新鲜子宫内膜组织,经单酶消化、二次筛网过滤及贴壁纯化技术分离纯化培养人子宫内膜腺上皮细胞和基质细胞,光镜及免疫细胞化学染色鉴定细胞纯度。结果:腺上皮细胞漩涡状生长,细胞呈蝌蚪形或类圆型,细胞角蛋白19免疫组化染色阳性,纯度可达92%。基质细胞呈平行状生长,细胞呈梭形或多角形。波形蛋白免疫组化染色阳性,纯度达95%以上。每例子宫肌瘤切除标本可获得(10～25)×106原代基质细胞和(4～6)×106原代腺上皮细胞。结论:该培养方法可获得高产量的纯化的子宫内膜腺上皮细胞和基质细胞。
Objective ：To establish a new simple method for isolating,purifying, and culturing human endometrial glandular epithelial and stromal cells.Methods：Enzymatic digestion, double filtration,and adherence purification were performed to obtain the endometrial glandular epithelial and stromal cells from the endometrial tissues of women undergoing total hysterectomy. These 2 types of cells were observed under hght microseope and identified by immunocytochemical staining. Results：The glandular epithelial cells grew in whorls and stained positively for cytokeratin 19 （CK19）, and the purity was above 92%. The stromal cells were spindle-shaped or polygonal-shaped and stained positively for vimentin, and the purity was above 95%. The numbers of stromal and glandular epithelial cells obtained from 1 endometrium sample were about 10×10^6 to 25×10^6 and 4×10^6 to 6×10^6, respectively. Conclusion：A better yield of purified human epithelial and stromal cells can be obtained by using this modified culture method.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2007年第5期608-610,共3页
Journal of China Medical University
