摘要
以豆腐渣和海带为原料水解制备水溶性膳食纤维(SDF)和多糖,并制备复合膳食纤维固体饮品。用碱法和酶法结合提取大豆不溶性膳食纤维(IDF)和SDF,采用复合酶法制备海带多糖。结果表明:大豆IDF的提取条件为氢氧化钠浓度5%﹑浸泡时间60min、浸泡温度80℃;纤维素酶添加量为IDF重量的1%,纤维素与水的比例为1:12(W/V),pH为4.5,水解时间为12h,水解温度为40℃,在以上条件下,SDF的产率为36.01%。提取海带多糖条件为加入海带粉重1%的复合酶,复合酶(纤维素酶、果胶酶和蛋白酶)的最佳配比为2:1:2,在pH6.0、50℃酶解6h总多糖得率最高,可达10.71%。
Water-solubility dietary fibcr（SDF） and kelp arnylose was prepared by hydrolysised byproduct of manufacturing bean curd and kelp, and akind of solid beverage of compound dietary fiber was manufactured. Soybean IDF（infusibility dietary fiber）and SDF were preared by the methods of alkali solution extracting and enzyme hydrolysis. Kelp amylose was prepared by hydrolysised with compound enzyme. The results showed that the extracted conditions of soybean IDF are the concentration of NaOH 5 %, soaking time 60 min and soaking temperature 80℃. The IDF are hydrolysised by cellulosic enzyme, and the conditions are the ratio of enzyme to substrate 1%, and ratio of IDF to water 1：12 （W/V）, pH value 4.5, hydrolysis time 12 hours, the temperature 40 ℃. Under the above conditions, the yield of SDF is 36.01%. The hydrolysis conditions for kelp amylose with compound enzyme are the ratio of compound enzyme to kelp powder 1%, the optimum proportion of the compound enzyme （cellulose, pectinase, protease） 2：1：2, and kelp is hydrolsised at pH 6.0 and 50℃ for 6h. The yield of kelp amylose is 10.71%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第4期172-176,共5页
Food Science
关键词
豆腐渣
膳食纤维
海带多糖
水解酶
byproduct of manufacturing bean curd
dietary fber
keln amvlose
hvdrolase
