摘要
目的探讨绝经后妇女血清基质金属蛋白酶-9(MMP-9)和基质金属蛋白酶抑制因子-1(TIMP-1)与骨密度(BMD)之间的关系。通过观察绝经后妇女不同骨密度条件下MMP-9、TIMP-1浓度的变化,探讨两者在骨质疏松症(OP)中的作用,为临床OP诊断与干预治疗提供依据。方法选择绝经后妇女80例。采用Challenge双能X线骨密度仪(DXA)测量腰椎(L2-L4)侧位和左侧髋部(股骨颈、大转子、Ward三角区)6个骨骼区域的骨密度(BMD)。分为正常对照组(骨密度正常组,21例)、低骨量组(20例)、骨质疏松组(23例)和严重骨质疏松组(骨质疏松骨折组,16例),对各组进行身高、体重等常规检查并用酶联免疫吸附试验(ELISA法)测定各组血清MMP-9和TIMP-1的浓度。结果绝经后女性血清MMP-9水平随骨密度的降低呈现升高趋势,以骨质疏松骨折组为著;低骨量组、骨质疏松组TIMP-1水平与对照组比较没有统计学意义;正常对照组、低骨量组、骨质疏松组MMP-9与TIMP-1的比率依次升高,致使MMP-9与TIMP-1的比率失调。结论绝经后妇女血清MMP-9水平升高及MMP-9与TIMP-1的比率失调可能为绝经后骨质疏松症发生的重要影响因素。
Objective To study the relationship between matrix metalloproteinase-9 （MMP-9）, tissue inhibitor of metalloproteinase- 1 （TIMP- 1 ） and bone mineral density（BMD） in postmenopausal women. By comparing the serum MMP-9 and TIMP-1 in different BMD conditions of postmenopausal women, to find out the function and variation of both in osteoporosis （OP） and to provide the data in the clinical diagnosis and treatment of OP. Methods Dual energy x-ray absorptiometry（DXA） was used to measure BMD of six skeletal regions of lumbar spine （L2-L4） and the left hip department （femoral neck, Ward＇s triangle and greater trochanter）. 80 postmenopausal women were divided into normal BMD group（ n = 21 ）, low BMD group（ n = 20）, osteoporosis group（ n = 23） and serious osteoprosis group（ osteoprosis bone fracture group, n = 16）. Each group was carried out the routine inspection such as height and weight, and measured serum MMP-9 and TIMP- 1 by ELISA. Results With the decreasing of BMD, the serum level of MMP-9 in postmenopausal women had an increased tendency. It was notable in osteoporosis bone fracture group; There was no statistical significance in serum density of TIMP-1 between low bone quantity group, osteoporosis group and normal comparing group; The ratio of serum MMP-9 and TIMP-1 of postmenopausal women was higher in three groups in turn, from the normal group to osteoporosis group. It causes the ratio imbalance between MMP-9 and TIMP-1. Conclusion The high level of MMP-9 and the ratio imbalance with the TIMP-1 could be an important factor for postmenopausal osteoporosis.
出处
《中国骨质疏松杂志》
CAS
CSCD
2008年第4期245-248,共4页
Chinese Journal of Osteoporosis
关键词
基质金属蛋白酶-9
基质金属蛋白酶抑制因子-1
双能X线骨密度仪
骨密度
骨质疏松症
绝经后
Matrix metalloproteinase-9（MMP-9）
Tissue inhibitor of metaUoproteinase-1 （TIMP-1 ）
Dual energy X-ray absorptiometry
Bone mineral density
Osteoporosis
Post-menopause
