摘要
为了探讨绝经后骨质疏松胶原代谢异常的分子机制 .采用RT PCR和Gel SDS 聚丙烯酰胺凝胶电泳(PAGE)分别检测卵巢切除大鼠骨组织Ⅰ型胶原mRNA水平及基质金属蛋白酶 (matrixmetalloproteinase,MMPs)的活性 ,免疫组化观察骨组织Ⅰ型胶原蛋白量 .卵巢切除大鼠骨组织Ⅰ型胶原mRNA表达降低约2 6 3% ,pro MMP 9活性明显提高 (P <0 0 5 ) ,应用雌激素治疗后Ⅰ型胶原mRNA表达较卵巢切除组增加34 1% ,pro MMP 9活性的增加明显降低 .卵巢切除后骨组织MMP 9明胶酶活性明显提高 ,应用雌激素后MMP 9明胶酶活性明显降低 (P <0 0 5 )表明骨Ⅰ型胶原mRNA表达减少和pro MMP
To Investigate the mechanism of postmenopausal osteoporosis in the metabolism of bone organic matrix,the levels of type Ⅰ collagen mRNA in ovariectomized rats bone tissue were detected by RT PCR. The activities of bone metalloproteinases(MMPs) were determined by Gel SDS PAGE.The amounts of type Ⅰ collagen in bone tissues were estimated by immuno histochemistry. The expression levels of type Ⅰ collagen mRNA decreased about 26 3%,and the activities of pro MMP 9 increased markedly in ovariectomized group compared with Sham group. Estrogen increased the levels of type Ⅰ collagen mRNA about 34 1% and obviously reduced the pro MMP 9 activities compared with OVX group. Ovariectomizing in rats resulted in the decrease of type Ⅰ collagen mRNA expression and the increase of pro MMP 9 activity,both contributed to the decrease of bone organic matrix and ovariectomized osteoprosis.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2001年第4期519-522,共4页
Progress In Biochemistry and Biophysics
