摘要
目的探讨乳黄片脑脊液对高氨环境中星形胶质细胞活力和胶质纤维酸性蛋白(GFAP)表达的影响。方法运用中药脑脊液药理学方法把体外培养的CRL-2541细胞分为空白对照组、氨损伤组、正常脑脊液(CSF)组、乳黄片脑脊液组,各组加入不同溶液。MTT检测CRL-2541活力,免疫细胞染色法检测GFAP表达情况。结果乳黄片脑脊液能升高高氨环境中受损细胞CRL-2541的吸光度,上调GFAP的表达。结论乳黄片能进入血-脑脊液屏障,对高氨环境中受损的星形胶质细胞有保护作用,提示其抗肝性脑病的作用位点可能为星形胶质细胞。
Objective To explore effects of ruhuang pill on the activity and GFAP expression of rat astrogia CRL-2541 cells under high ammonia. Methods The cultured CRL-2541 cells were divided into control group, amonia injured group, normal CSF group, CSF group and Ruhuang pill group. Different solutions were used in each group. The activity of CRL-2541 was detected by MTT test and the expression of GFAP was analyzed by immunologic cell staining method. Results The CSF of ruhuang pill increased the absorbanee of CRL-2541 cells injured under the high ammonia and up-regulated the GFAP expression. Conclusion Ruhuang pill could enter in BBB and protect the injured astroglia in high ammonia, which suggests that the target of anti-liE would lie in astroglia.
出处
《医药导报》
CAS
2008年第4期371-373,共3页
Herald of Medicine
基金
湖北省自然科学基金资助项目(基金编号:2002AB120)
