摘要
目的通过观察当归芍药散精简方(FBD)含药脑脊液对不同因素所致的PC12细胞损伤模型的保护作用,来探讨当归芍药散精简方(FBD)防治痴呆的作用机理。方法以MTT法观察FBD含药脑脊液对谷氨酸、过氧化氢、连二亚硫酸钠和氯化钾造成的PC12细胞损伤模型中细胞存活率的影响。结果连续多次给药(1.62g生药·kg-1)后0.5h～2.5h的FBD含药脑脊液对过氧化氢损伤的PC12细胞均有明显保护作用(P<0.05～0.01),其中以1.5h的FBD含药脑脊液保护作用最明显;该时间点FBD含药脑脊液对谷氨酸、连二亚硫酸钠和氯化钾造成的PC12细胞损伤模型亦有显著保护作用,且随脑脊液添加量增加保护率捉高。结论FBD含药脑脊液对PC12细胞损伤有显著保护作用,其机制与抑制细胞内钙超载、对抗自由基的氧化损伤和谷氨酸的兴奋性毒性等有关。
Objective： Investigating the protective effect of CSF of rat administrating FBD orally on various injured PC12 cells models to indicate the mechanism of FBD therapying dementia. Methods： Four injured PC12 models induced by hydrogen peroxide, hydrosulfurous sodium, glutamate and potassium chloride accordingly, were used to assay the effect of CSF sample on PC12 cells. Results：It was found that the rat CSF sample at different time points from 0.5h to 2.5h after taking FBD repetitiously （1.62g crude herb·kg^-1） could obviously increase the viability of PC12 cells injured by hydrogen peroxide,which peaked at 1.5h.The rat CSF containing FBD of 1.5h could also protect PC12 cells injured by hydrosulfurous sodium,glutamate and potassium chloride, and the protection rate increases with the concentration of CSF containing FBD. Conclusion： CSF of rat administrating FBD orally could protect PC12 cells, possibly by antagnizing the overload of calcium, oxidant injury of free radicals and excitatory nerotoxicity of glutamate.
出处
《中国实验方剂学杂志》
CAS
2005年第5期55-58,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家新药基金资助项目(969010538)
国家自然科学基金资助项目(30271604)
