摘要
建立了高效液相色谱法（HPLC）测定竹节参中人参皂苷Rg1、Re、Rb1、Rb2、Rg2、Rd含量的方法．运用二极管阵列检测器（DAD）峰纯度和光谱检索功能，结合保留时间定性，外标峰面积法定量．采用C18反相柱，以乙腈-水梯度洗脱测定了同一批竹节参总皂苷中人参皂苷Rg1、Re、Rd的含量分别为0．81％、0．15％、2．99％，回收率为93．46％-94．02％，含量及回收率的RSD均小于5％，该方法简便、灵敏，精密度及准确度在允许范围内，可作为竹节参皂苷提取物中多种人参皂苷的同时测定方法．
A HPLC method for determination of ginsenosides including Rg1, Re,Rb1, Rb2, Rg2, Rd in panax japonicus C. A. Mey was reported. HPLC analysis was performed on a C18 reversed-phase column （5 μm ,4. 6 mm×250 mm）using mobile phase of acetonitrile and water mixture under gradient elution. Ginsenosides Rg1,Re,Rd in a bath of chilkusetsusaponin extract were tested . The contents of Rg1, Re, Rd were 0.81% , 0. 15%,2. 99% respectively and the recovery was 93.46%-94. 02%. Both sides relative standard deriations were less than 5%. This method is simple, sensitivity, reproducibility and can be used for the determination of ginsenosides in panax japonicus C. A. Mey by HPLC.
出处
《分析测试技术与仪器》
CAS
2008年第1期19-22,共4页
Analysis and Testing Technology and Instruments
