摘要
【目的】探讨不同浓度骨保护素(osteoprotegerin,OPG)对破骨细胞(osteoclasts,OC)生成和活化的影响。【方法】通过成骨细胞(osteoblasts,OB)与脾细胞共培养的方法在体外诱导脾细胞转化为OC。采用形态学观察、抗酒石酸酸性磷酸酶(TRAP)染色计数、扫描电镜观察象牙片吸收陷窝方法检测OC的生成和活化。【结果】随着OPG浓度(5、10、25、50、75ng·ml-1)的增加,TRAP阳性OC数逐渐减少,各试验组与对照组比较差异均极显著(P<0.01);象牙片吸收陷窝的数目和面积也逐渐减少,OPG浓度为50ng·ml-1、75ng·ml-1时观测不到吸收陷窝。【结论】OPG通过抑制OC生成和活化,从而抑制OC的骨吸收,OPG浓度达50ng·ml-1以上可完全阻断OC的活化。
【Objective】To study the effect of different concentration of OPG on osteoclasts. 【Method】Osteoclasts were obtained by a co-culture system of osteoblasts with rat spleen cells, and supplemented with 5, 10, 25, 50 or 75 ng·ml-1 OPG, respectively. The formation and activation of osteoclasts were detected （or examined） by ways of morphologic observation, histochemical staining for tartrate-resistant acid phosphatase （TRAP） and detection of lacunar resorption through scanning electron micrograph. 【Results】Compared with the control group, the number of TRAP positive osteoclasts in experiment group decreased very significantly （P〈0.01）. The number and area of lacunar resorption decreased with concentration increase of OPG. Lacunar resorption wasn’t detected in the group, which with more than 50 ng·ml-1 OPG. 【Conclusion】The resorption activity of osteoclasts is inhibited by inducing osteoclasts formation and activation via OPG.
出处
《中国农业科学》
CAS
CSCD
北大核心
2008年第2期581-586,共6页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目(30440050,30571364)
江苏省高校自然科学研究指导性计划项目(05KJD230074)
江苏省高校“青蓝工程”优秀青年骨干教师资助项目(苏教师[2005]12)
江苏畜牧兽医职业技术学院院内课题(200503)
关键词
破骨细胞
骨保护素
抗酒石酸酸性磷酸酶
吸收陷窝
Osteoclast
Osteoprotegerin
Tartrate-resistant acid phosphatase
Lacunar resorption
