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体外培养破骨细胞TRAF6的表达 被引量：1

Expression of TRAF6 in osteoclasts formation in vitro
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摘要 目的:观察体外培养破骨细胞过程中肿瘤坏死因子受体相关因子6(TRAF6)的表达,探讨其与破骨细胞功能的相关性。方法:应用1, 25(OH)2D3 体外诱导大鼠骨髓细胞形成破骨细胞,观察记录抗酒石酸酸性磷酸酶(tartrate-resistantacidphosphatase, TRAP)阳性多核巨细胞的数目,并采用免疫组织化学的方法检测TRAF6的表达。结果:应用1, 25(OH)2D3 的实验组可见TRAF6的阳性表达,并随培养时间的延长发生表达强度的变化。结论:TRAF6可能参与了体外培养破骨细胞的成熟与分化。 AIM:To observe the expression of tumor necrosis factor - associated factor 6(TRAF6) in the formation of osteoclasts in vitro and study the relation between TRAF6 and osteoclasts.METHODS:Marrow cells from SD mouse were harvested and cultured in the culture system with 1,25(OH) 2D 3.The appearance of tartrate-resistant acid phosphatase(TRAP)-staining multinuclear cells were measured.The effect of the expression of TRAF6 in murine narrow cell was examined by method of immunohistochemistry.RESULTS:TRAF6 positive signals were observed in osteoclasts in vitro.CONCLUSION:These results demonstrate that the TRAF6 is involved in the differentiation and mature of osteoclasts in vitro.
作者 张娜 杨富生 刘艳丽 徐建立
机构地区 第四军医大学口腔医学院
出处 《牙体牙髓牙周病学杂志》 CAS 2005年第4期189-192,共4页 Chinese Journal of Conservative Dentistry
关键词 肿瘤坏死因子受体相关因子6 破骨细胞 1 25-二羟维生素D3 tumor necrosis factor receptor-associated factor osteoclast 1,25(OH)2D3
分类号 R780.2 [医药卫生—口腔医学]
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参考文献10

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共引文献11

同被引文献9

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牙体牙髓牙周病学杂志
2005年 第4期

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