摘要
对直接从鸭骨髓腔机械分离的成熟破骨细胞(osteoclasts,OC)和由鸭骨髓来源单核细胞融合成的OC样多核巨细胞(multinucleated giant cells,MNGCs)进行培养,分别进行抗酒石酸酸性磷酸酶(tartrate-resistant acid phospha-tase,TRAP)染色并计数,扫描电镜观察象牙片吸收陷窝,比较了2种方法获得OC的骨吸收功能。结果显示,2种方法均能分离培养出TRAP阳性且具有骨吸收功能的多核OC,但直接分离获得的成熟OC骨吸收功能更强。
Mature osteoclasts （OC） separated from duck marrow and OC-like multinucleated giant cells （MNGCs） fused from bone marrow-derived mononuclear were cultured. Then, they were stained by tartrate-resistant acid phosphatase （TRAP） at different times and weve counted. The activity of resorption were detected by scanning electron microscopy on ivory slices. The results showed that, TRAP-positive multinucleated OC can be harvested with both methods, while mature OC isolated directly had more ability of bone resorption.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第11期1708-1711,1729,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30972229
30571364)
江苏省自然科学基金资助项目(BK2008214)
关键词
破骨细胞
抗酒石酸酸性磷酸酶
象牙片
骨吸收
osteoclasts
tartrate-resistant acid phosphatase
ivory slices
bone resorption
