摘要
目的：探讨亮－脑啡肽（L－Enk）抗体在纹状体、下丘脑、中脑和海马对大鼠氧惊厥的影响。方法：120只大鼠被随机分为12组，先在纹状体、下丘脑、中脑和海马分别注射微量L－Enk抗体或生理盐水，然后将动物暴露在0．6MPa（6ATA）高压氧（HBO）下，观察各组大鼠发生氧惊厥的潜伏期、惊厥发作的程度及动物的存活时间。结果：在纹状体和下丘脑，与对照组相比能够显著延长大鼠发生氧惊厥的潜伏期，减轻惊厥发作的程度和延长动物存活的时间（P＜0．05～0．01）；而中脑和海马与对照组比则相差不显著（P＞0．05）。结论：在大鼠纹状体和下丘脑分别注入微量L－Enk抗体，对氧惊厥具有一定的预防作用，提示大鼠氧惊厥的发生与纹状体和下丘脑内的L－Enk含量相关。
Aim: To investigate the effects of injection of Leu-Enkephalin (L-Enk ) antibody into striatum, hypothalamus, mesencephalon and hippocampus on oxygen convulsions in rats. Methods: One hundred and twenty male SD rats were randomly divided into 12 groups. L-Enk antibody(0. 5 μl or 1. 0μl)or saline were dricro-injected into striatum, hypothalamus, mesencephalon and hippocampus respectively in different groups before the animals were exposed to 0. 6 MPa (6 ATA) hyperbaric oxygen (HBO). The latent period of convulsions (LPC),the severity of convulsions (SC) and the survival time (ST) of animals in each group were observed' Results:As compared with respective control groups, the LPC was significantly prolonged,the SC was significantly mitigated and the ST was significantly increased in the striatum and hypothalamus experiment groups (Pr0. 05~0.01 ),while there were no significant difference in the mesencephalon and hippocampus experiment groups (P> 0. 05 ). Conclusion:There were considerable oxygen convulsions preventive effect with the micro-injection of L-Enk antibody into striatum and hypothalamus. The results suggested that the oxygen convulsions of rats were related to the contents of L-Enk in striatum and hypothalamus.
基金
国家自然科学基金!38970319
关键词
氧惊厥
氧中毒
脑啡肽
抗体
Enkephalin Hyperbaric oxygen Convulsions
