摘要
目的 :探讨狗颅脑爆炸伤后脑组织中 TNF-α m RNA的变化及其规律。方法 :应用原位杂交技术分别检测狗颅脑爆炸伤后 30 min和 1、3、6 h大脑皮质、海马、下丘脑和脑干等不同脑区脑组织中 TNF-α m RNA的表达情况 ,并与正常对照组比较。应用电子计算机图像分析技术对其结果进行半定量分析。结果 :正常对照组狗脑组织内 TNF-α m RNA即有表达 ;颅脑爆炸伤后不同脑区脑组织中 TNF-α m RNA的表达量均明显升高 ,变化幅度以大脑皮质挫伤区和同侧海马最为显著。 TNF-αm RNA在伤后 30 m in表达量即明显增加 ,至伤后 1h达到高峰 ,伤后 3h呈下降趋势 ,但仍高于正常水平。伤侧大脑半球TNF- α m RNA的表达量明显高于对侧大脑半球。结论 :TNF- α m RNA在生理状态下即有表达 ,TNF- α参与正常的生理功能 ;颅脑爆炸伤后早期脑组织中 TNF- α的表达增多 ,提示 TNF-
Objective: To investigate the expression changes of TNF α mRNA after brain explosive injury in dogs. Methods: With the brain explosive injury model we had developed, nuclei acid in situ hybridization with an antisense RNA probe and computer image analysis were used to detect the dynamic changes of TNF α mRNA in the cerebral cortex, hippocampus, hypothalamus, and up brain stem after brain injury. Results: The expression of TNF α mRNA could be found in several regions of normal dog's brain tissues, and the expression became more obvious and widespread following explosive injury to the brain. In all of the brain regions, the expression changes of cerebral contussive area and hippocampus were more significant. TNF α mRNA expression increased significantly at 30 min and reached maximum at 1 h after the explosive injury, and then decreased gradually, which was higher than normal's. More expression were detected in the ipsilateral hemisphere than contralateral hemisphere. Conclusion: The expression of TNF α mRNA in basal condition implicate that TNF α may play an important role in neural function. It is suggested that TNF α may play an important role in traumatic brain injury. [
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2001年第6期551-553,F003,共4页
Academic Journal of Second Military Medical University
基金
全军"九五"医学科研计划指令性研究资助项目 (96L0 3 6)
