摘要
目的：确切评估动员后外周血干细胞（ＰＢＳＣ）水平的变化，及时指导临床选择最佳采血时机。方法：用流式细胞术测定化疗和粒细胞集落刺激因子（Ｇ－ＣＳＦ）联合动员时外周血ＣＤ３４＋Ｔｈｙ－１＋细胞含量的变化，同时用体外集落培养方法评价外周血祖细胞（ＰＢＰＣｓ）的克隆形成能力。结果：动员后循环血中ＣＤ３４＋Ｔｈｙ－１＋细胞、ＣＤ３４＋细胞和克隆形成细胞（ＣＦＣ）含量分别增高４８．６倍、５０．０倍和５３．１倍，高峰时间在化疗后第１２～１４天（注射Ｇ－ＣＳＦ的第６～８天）；外周血单个核细胞中ＣＤ３４＋Ｔｈｙ－１＋细胞、ＣＤ３４＋细胞的比例分别增高１３．８倍和１０．５倍；动员的早期阶段，ＣＤ３４＋细胞中Ｔｈｙ－１＋细胞比例最高。结论：联合应用化疗和Ｇ－ＣＳＦ对ＰＢＰＣｓ，尤其对早期干／祖细胞具有显著动员作用；用流式细胞术检测ＣＤ３４＋Ｔｈｙ－１＋细胞可及时指导临床准时采集ＰＢＳＣ。
Objective:To estimate the amount of peripheral blood stem cells(PBSC) right during mobilization and determine the optimal time for PBSC collection.Methods:A serial measurement of circulating CD 34 + Thy 1 + cells were carried out during PBSC mobilization by chemotherapy and granulocyte colony stimulating factor(G CSF) using dual color direct immunofluorescence flow cytometry. Colony culture in vitro was performed at the same time to evaluate the clonogenic capacity of the peripheral blood progenitor cells (PBPCs).Results:Circulating CD 34 + Thy 1 + cells,CD 34 + cells and colony forming cells increased by 48.6 ,50 and 53.1 fold, respectively,after mobilization.The peak levels were reached at 12～14 days after chemotherapy.The proportion of CD 34 + cells and the candidate stem cells,CD 34 + Thy 1 + subset,in mononuclear cells(MNCs) exceeded baseline by 10.5 and 13.8 fold, respectively.The percentage of CD 34 + cells coexpressing Thy 1 was highest in the earlist phase of mobilization.Conclusion:PBSCs,especially primitive progenitor cells,increased significantly during mobilization with chemotherapy and G CSF.Flow cytometric assay of circulating CD 34 + Thy 1 +cells promptly provides a more reliable indicator for clinical collection of PBSC.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1997年第8期406-409,共4页
Chinese Journal of Hematology
关键词
造血干细胞移植
外周血
干细胞动员
Hematopoietic stem cell Peripheral blood stem cell mobilization
