摘要
目的探讨大鼠部分肝移植后骨髓干细胞动员的情况。方法建立大鼠性别交叉部分肝移植模型,分为部分肝移植组、全肝移植组和假手术组,分别与术后1、3、5、7 d取标本。用流式细胞法检测骨髓中干细胞标记的细胞群体的数量变化,荧光原位杂交检测移植肝脏内Y染色体特异的Sry基因的表达, 免疫组织化学法检测移植肝脏内干细胞标志CD34,c-kit和Thy-1.1的表达。结果与全肝移植组相比, 部分肝移植组术后第1天,骨髓细胞中,β2微球蛋白(β2m)/Thy-1.1+,CD45+/CD34+有不同程度的升高,然后呈递减趋势。免疫组织化学检测汇管区和炎性灶周围可见CD34、c-kit和Thy-1.1单个核细胞表达,并于第5至7天后减少。同时免疫组织化学双染可检出CD34+/CD45+细胞。全肝移植组汇管区CD34、c-kit和Thy-1.1表达较少,假手术组CD34、c-kit和Thy-1.1偶见表达。在部分肝移植物可检出Sry+细胞,但Srg+/CD34+,Sry+/Thy-1.1+细胞较少。全肝移植组Sry+偶见。结论部分肝移植中,骨髓源性的干细胞发生动员,同时肝内有干细胞被激活,其中外源性干细胞较少。
Objective To explore the role of stem cell mobilization on regeneration of partially grafted livers. Methods Rats models with cross-sex 50% PLTx （partial liver transplantation） were established. The rats were divided into three groups： PLTx, WLTx （whole liver transplantation） and sham operation groups. Bone marrow and liver samples were collected on days 1, 3, 5, 7 postoperatively （each n = 6）. The quantitative variations of the cells with stem cell markers in the bone marrow, including β 2m/Thy-1. 1^＋, CD45^＋/CD34^＋, Fit2/3^＋ and c-kit^＋ markers, were detected using flow cytometry. Sty gene positive cells in donor livers were detected by fluorescent in situ hybridization （FISH）, and the expressions of CD34, c-kit and Thy-1.1 were detected by immunohistochemistry technique. Results Compared with the WLTx and sham operation groups, β2m /Thy-1.1^＋, CD45^＋/CD34^＋ cells in bone marrows in the PLTx group increased on the first postoperative day and decreased on the following days. The CD34, c-kit and Thy-1.1 positive cells detected in portal tract areas peaked during the 3-5 postoperative days. CD34^＋/CD45^＋ positive cells could be detected. The expressions of CD34, c-kit and Thy-1.1 positive cells were rare in the WLTx and sham operation groups. Sty^＋ cells could be detected in portal tract areas and few Sry^＋/CD34^＋ and Sry^＋/Thy-1,1^＋ cells were detected. Conclusion In the PLTx group, the stem cells in the bone marrow were mobilized and stem cells in the liver were activated.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2005年第11期839-843,共5页
Chinese Journal of Hepatology
基金
国家"863"计划(2001AA216031)高等学校博士学科点专项科研基金(20040001133)
