摘要
目的:探讨刺五加对小鼠睡眠剥夺模型免疫功能和抗疲劳能力的影响。方法:将小鼠随机分为正常对照组、模型组和刺五加低、高剂量组。刺五加低、高剂量组分别按照500,1 000 mg·kg-1的剂量ig,连续给药10 d,正常对照组和模型组每天ig等体积的蒸馏水。在第11 d,将模型组和刺五加低、高剂量组的小鼠采用单平台水环境法进行睡眠剥夺(sleepdeprivation,SD),在SD期间,继续给予实验药物。SD连续3 d后,采用炭粒廓清实验观察药物对SD小鼠免疫功能的影响,通过负重游泳实验和转棒疲劳实验观察药物对SD小鼠抗疲劳能力的影响。结果:炭粒廓清实验中,刺五加500,1 000 mg·kg-1的K值和α值为(0.021 4±0.008 3,0.030 7±0.005 9),(4.0±0.2,4.6±0.2),与SD组相比均显著增加;抗疲劳实验中,刺五加500,1 000 mg·kg-1的游泳时间和转棒时间为(828±83),(990±64)S,(330±63),(665±170)s,与SD组相比均显著延长。结论:刺五加具有增强睡眠剥夺小鼠免疫功能和抗疲劳能力的作用。
Objective： To study the effects of Acanthopanax senticosus （500, 1 000 mg ·kg^-1） on immune function and anti-fatigue ability in sleep deprivated mice. Method： Sleep-deprivated mice model was induced by single platform technique, the immune function of sleep-deprivated mice was tested by the clearance rate of charcoal particles and the level of serum, and anti-fatigue ability was measured by the weight-loaded swimming method and the rotated performance test. Result： A. senticosus could significantly enhance the clearing index and the correction clearing index of sleep deprivated mice, the time of loaded-swimming and the resideece time on the rotated sticks deprivation mice were extended significantly. Conclusion： A. senticosus can increase immune functions and anti-fatigue ability of sleep deprivated mice
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第23期173-175,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
福建省自然科学基金青年创新项目(2011D016)
关键词
刺五加
睡眠剥夺
免疫
抗疲劳
Acanthopanax senticosus
sleep deprivation
immune function
anti-fatigue
