摘要
首先以人γ－免疫球蛋白为实验物系，对Ａ蛋白中空纤维亲和膜的吸附性能进行了考察．然后应用亲和膜，从小鼠腹水上清液中直接吸附小鼠抗人绒毛膜促性腺激素单克隆抗体．纯化的单克隆抗体经ＳＤＳ－聚丙烯酰胺凝胶电泳检测达到电泳纯．
Adsorption properties of protein A hollow fiber affinity membrane are investigated using human γ immunoglobulin as the model protein. For practical application, anti human chorionic gonadotropin monoclonal antibody is purified from mouse ascites. The antibody purified is pure as judged by SDS polyacrylamide gel electrophoretic analysis.
出处
《膜科学与技术》
CAS
CSCD
1997年第4期21-24,共4页
Membrane Science and Technology
基金
国家自然科学基金
关键词
吸附
单克隆抗体
中空纤维膜
A蛋白
膜分离
affinity membrane adsorption human chorionic gonadotropin monoclonal antibody
