摘要
以纤维素滤纸为载体,通过环氧氯丙烷交联活化、亚氨基二乙酸偶联、金属离子螯合制备得到亲和膜。应用该膜进行的蛋清蛋白纯化试验结果表明,Cu2+亲和膜具有较好的吸附性能和纯化效果,只需采用两步洗脱的方式即可得到具有明显纯化效果的蛋清转铁蛋白和溶菌酶。
Affinity membrane was successfully prepared with cellulose analysis filter paper as support, epichlorohydrin（EPI） as crosslinking and activation agent,iminodiacetic acid （IDA）as ligand. Then preparation of Ni^2＋, Zn^2＋, Cu^2＋ metal- chelated ligand was carried out, respectively. The experimental results showed that the absorption and purification effect of Cu^2＋ affinity membrane were better than those of Ni^2＋ and Zn^2＋ affinity membranes. The ovotransferrin and lysozyme were purified as salience with two step elution. The affinity membrane supplied a technique for industrilized membrane separation in the future.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2006年第2期42-47,共6页
Journal of Food Science and Biotechnology
关键词
亲和膜
纤维素
卵转铁蛋白
溶菌酶
affinity membrane
cellulose ovotransferrin（OTf）
lysozyme（Lys）
