摘要
比较了黑曲霉(AS3.350)以有机和无机氮为氮源液态发酵制备果胶酯酶的效果,并对有机氮源的液态发酵条件进行优化。结果表明,以有机氮为氮源的发酵效果优于无机氮,黑曲霉(AS3.350)接种量0.3mL(3×103个孢子),转速90r/min,30℃发酵4d时,果胶酯酶活力高达93.4U/mL培养基。
Production ofpectinesterase （PE） by Aspergillus niger （AS3.350） was studied in submerged fermentation systems. As peptone is a sole nitrogen source, PE production in submerged fermentation systems was higher than the production in systems with NH4NO3 as a sole nitrogen source. After studying the fermentation conditions of A. niger （AS3.350）, the maximum activity of PE （93.4U/ml） was detected during inoculums 3×10^3 spores, 90r/min in an orbital shaker, incubation at 30℃ for 4 d.
出处
《中国酿造》
CAS
北大核心
2007年第12期28-31,共4页
China Brewing
关键词
黑曲霉
果胶酯酶
发酵条件
Aspergillus niger
pectinesterase： fermentation conditions
