摘要
从361株细菌和酵母菌中分别筛选到12株可不对称水解布洛芬乙酯生成R(-)-布洛芬的细菌(5株菌对映体过量(ee)可达85%)和15株具有不对称水解布洛芬乙酯活性的酵母菌,其中皮状丝孢酵母T158生成S(+)-布洛芬,ee可超过92%.该酵母菌最适碳源为葡萄糖,浓度以1.0—1.5%适宜,蛋白胨浓度低于0.3%或高于0.5%对水解拆分均不利.酵母膏的加入显著提高水解活性,最适浓度0.3%.在培养基中添加表面活性剂吐温80(0.2%)既可提高拆分专一性,又能增强水解能力.
Microorganisms that hydrolyze (±)-ethyl-2-(4-isobutylphenyl) propionatewere screened from 361 strains. 12 strains of bacteria preferentially hydrolyzed (R)-isomer of the racemic ester to produce R(-)-2-(4-isobutylphenyl)propionic acid. Five ofthem showed higher specificity of resolusion about 85% ee. 15 strains of yeasts have theactivity of resolution. Among others, the Trichosporon cutaneum 158 preferentiallyhydrolyzed (S)-isomer to produce S(+)-2-(4-isobutylphenyl)propionic acid with 92%ee. Adding tween 80 in the liquid medium (pH 6.5)can improve the specificity ofresolution and increase the activity of hydrolysis obviously.
出处
《微生物学报》
CSCD
北大核心
1995年第3期190-196,共7页
Acta Microbiologica Sinica
基金
国家重点科技攻关项目部分工作
关键词
皮状丝孢酵母
不对称水解
布洛芬
合成
Trichosporon cutaneum
Asymmetric hydrolysis
S ( + )-2-( 4-isobutylphenyl)propionic acid
