摘要
目的:探讨半枝莲提取物(Scutellaria barbataextract,SBE)对人肺癌SPC-A-1细胞凋亡及凋亡相关基因survivin和caspase-3表达的影响。方法:体外培养肺癌SPC-A-1细胞,以2.5、5、10 mg/L SBE处理SPC-A-1细胞48h,并以2.5 mg/L顺铂(DDP)作为阳性对照。用MTT法检测细胞抑制率,TUNEL法检测凋亡率,半定量RT-PCR检测survivin和caspase-3 mRNA表达。结果:与正常对照组相比,SBE各组细胞抑制率显著上升(P<0.001),细胞凋亡率明显升高(P<0.01),Caspase-3 mRNA表达显著上升(P<0.05或P<0.01),Survivin mRNA表达明显下降(P<0.05或P<0.01)。结论:SBE具有诱导肺癌SPC-A-1细胞凋亡的作用,其分子机制可能与上调caspase-3及下调survivin基因表达有关。
Objective ： To explore the apoptosis-inducing effect of Scutellaria barbara extract （SBE） and the expression of apopto- sis associated genes survivin and caspase-3 on human lung cancer SPC-A-1 cells. Methods： Lung eancer SPC-A-1 cells were treated with 2. 5,5 and 10 mg/L for 48 h,and the cells were treated with 2.5 mg/L DDP as positive control. The inhibitory rat was evaluated by MTT assay. Apoptotic rate was determined by TUNEL method. The expression of survivin and caspase-3 mRNA were detected by semi-quantitive RT-PCR. Results ： Compared with control group, the inhibitory rate was increased obviously （ P 〈 0.001 ）, the apoptotic rate was increased markedly （ P 〈0. 01 ） , the expression of caspase-3 mRNA was increased significantly （ P 〈 0. 05 or P 〈 0. 01 ） , while survivin mRNA was decreased markedly （P 〈0. 05 or P 〈0. 01 ） in SBE groups. Conclusion： SBE can induce apoptosis of SPC- A-1 cells. The molecular mcchanism may be related to up-regulating expression of easpase-3 and down-regulating expression of survivin genes.
出处
《中药材》
CAS
CSCD
北大核心
2007年第10期1270-1273,共4页
Journal of Chinese Medicinal Materials
