摘要
目的探讨花边莲提取液对人肺癌SPC-A-1细胞凋亡相关基因survivin和caspase-3表达的影响。方法采用体外培养人肺癌SPC-A-1细胞,实验随机分为正常对照组、花边莲处理组(浓度分别为12.5,25和50 m l/L)和顺铂(DDp25mg/L)组,半定量RT-PCR检测survivin和caspase-3 mRNA表达水平,二步法免疫组化检测Survivin和Caspase-3蛋白表达变化。结果与正常对照组相比,花边莲各浓度组caspase-3 mRNA和蛋白表达显著上升(P<0.01),survivin mRNA和蛋白表达明显下降(P<0.01)。结论花边莲提取液诱导SPC-A-1细胞凋亡的分子机理可能与上调caspase-3基因表达和下调survivin基因表达有关。
Objective To investigate the influence of HuaBianLian（HBL） extract on expression of apoptosis associated genes survivin and caspase-3 in human lung cancer SPC-A-1 cells. Methods Cultured human lung cancer SPC-A-1 cells were randomly divided into the control group （ the negative control group）, HBL - treated groups（ 12.5,2.5,50 ml/L） and DDP（25 mg/L） group （the positive control group）. The expression of survivin and caspase-3 mRNA was detected by semi-quantitive RT-PCR . The expression of survivin and caspase - 3 proteins were detected by two-step immunhistochemical staining. Results Compared with control group , the expression of caspase -3 mRNA and protein increased significanty （ P 〈 0.01 ）, while survivin mRNA and protein decreased markedly in HBL groups （ P 〈 0.01 ）. Conclusion The molecular mechanism of HBL apoptosis -inducing of SPC-A-1 cells may be associated with the up-regulating expression of caspase - 3 gene and down-regulating expression of survivin gene.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第1期17-19,共3页
Lishizhen Medicine and Materia Medica Research
基金
江西省卫生厅中医药科研基金课题资助项目(No.200133)
江西省教育厅课题资助项目(No.88B03)
