摘要
目的:建立山茱萸配方颗粒中马钱苷的含量测定方法.方法:色谱柱为HangBang C18(200*4.6mm5μm):流动相为乙腈:水(15:85);检测波长240nm;流速1.0ml/min;柱温30℃.结果:马钱苷的检测浓度线性范围为0.005～0.025μg(r=0.9998),RSD=2.16%(n=5);平均加样回收率为98.79%,RSD=1.77%(n=6).结论:本法简便、快捷、准确,可用于山茱萸配方颗粒的含量测定.
Objective：To establish a HPLC method for dispensing granule of Comus officina/is Sieb.et Zucc. Methods：The HPLC system consisted of HangBang C18（200 ＊ 4.6mm5μm）,Acetonitrile - water（15：85）mixture as a mobile phase,detection wavelength at 270nm and 1.0ml/min of flow rate.Results：The calibration curve of loganin was linear in the concentration range of 0.005-0.025μg（ r= 0.9998）, RSD= 2.16%（n= 5） ;The average recovery was 98.79 %, RSD = 1.77 % （n = 6）. Conclusion ：This method is simple, quick and specific, and suitable for the quality control of dispensing granule of Comus officinalis Sieb.et Zucc.
出处
《黑龙江医药》
CAS
2007年第6期554-555,共2页
Heilongjiang Medicine journal
关键词
山茱萸配方颗粒
马钱苷
高效液相色谱法
dispensing granule of Comus officinalis Sieb.et Zucc
loganin
HPLC
