摘要
目的:建立以高效液相色谱法测定复方利血平片中利血平含量的方法。方法:色谱柱为phenomenexC18,流动相为乙腈-水(60∶40),检测波长为268nm,流速为1.0mL·min-1,进样量为20μL。结果:利血平检测浓度的线性范围为1.93～38.60μg·mL-1(r=1.0000);平均回收率为98.9%(RSD=1.06%)。结论:本方法操作简便、快速、准确,可用于该制剂的质量控制。
OBJECTIVE： To establish HPLC method for the determination of the content of reserpine in compound reserpine tablets. METHODS： The determination was performed on phenomenex C18 column. The mobile phase consisted of acetonitrile water （60 ： 40） at a flow rate of 1.0mL · min^-1. The UV detection wavelength was set at 268nm and the sample size was 20μL. RESULTS： The linear range of reserpine was 1.93-38.60μg ·mL ^-1（r = 1.000 0）, with average recovery at 98.9% （RSD = 1.06%） .CONCLUSION： The established method is simple, rapid and accurate, and suitable for the quality control of compound reserpine tablets.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第25期1978-1980,共3页
China Pharmacy
