摘要
目的观察首乌丹参方(GTSMP)对大鼠心肌缺血再灌注损伤(I/R)保护作用。方法将实验大鼠分为假手术组、模型组、首乌丹参方高剂量组(9g生药/kg)、首乌丹参方中剂量组(4.5g生药/kg)、首乌丹参方低剂量组(2.25g生药/kg)、阳性对照药复方丹参滴丸组(4.5g生药/kg)、工具对照药消心痛组(1.67mg/kg),共7组。采用结扎大鼠冠状动脉前降支30min/开放120min建立心肌I/R模型。通过RT-PCR分子生物学方法,观察蛋白激酶C(PKC) mRNA和iNOS mRNA的表达情况以及首乌丹参方预处理对其的影响。结果与假手术组相比,模型组和首乌丹参方各组PKC mRNA表达均有所下降;与模型组相比,首乌丹参方中剂量组、复方丹参滴丸组和消心痛组表达上调,均表现出显著性差异,首乌丹参方高剂量组及低剂量组也能促进PKC mRNA的表达,但没有显著性差异;假手术组心肌iNOS mRNA弱表达,模型组呈现高表达,首乌丹参方可下调iNOS mRNA基因表达。结论首乌丹参方预处理可促进I/R的大鼠心肌的PKC表达,下调I/R大鼠心肌iNOS mRNA的表达;其通过激动PKC,使心肌组织iNOS mRNA弱表达,可能是首乌丹参方对缺血再灌注损伤的心肌的保护效应机制之一。
Objective To observe the protective mechanism against experimental myocardial ischemia-reperfusion rats pretreated with Gold Theragran Salvia miltiorrhiza prescription （GTSMP）. Methods The rats were divided into seven groups： sham group, model group, GTSMP high-dose group（9g/kg）, moderate-dose group（4.5/kg） and low-dose group （2.25/kg）, Isorbil （1.67mg/kg） and compound Danshen dripping pill （4.5g/kg） as control groups. The rat model of IR was built by ligating the left anterior descending coronary, artery for 30 minutes followed by 2 hours of reperfusion, to observe the expression mRNA of protein kinase C and iNOS and effect of pretreated with GTSMP to I/R rats by the method of RT-PCR. Results The expression of PKC mRNA of model group and every GTSMP group was down-regulated compared with that of sham operation group. Compared with model group, the expression of PKC mRNA of GTSMP moderate dose treated group, compound Dan-shen dripping pill treated group and Isorbil group was up-regulated significantly. GTSMP high-dose group and GTSMP low-dose group can also promote the expression, but they did not have significant differences. The expression of iNOS mRNA in model group was up-regulated compared with sham group and in every GTSMP group; the expression of iNOS mRNA was down-regulated compared with model group. Conclusion GTSMP pretreatment can up-regulate the expression of protein kinase C-mRNA and down-regulate iNOS mRNA on IR in rats. The possible protective mechanism may up-regulate expressions of the protein kinase C-mRNA and down- regulate the expression of iNOS mRNA.
出处
《上海中医药杂志》
北大核心
2007年第10期75-78,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
天津市科技发展基础研究重点资助项目(033802111)
天津市高等学校科技发展基金资助项目(20030101)
关键词
首乌丹参方
缺血再灌注
蛋白激酶-C
诱生型一氧化氮合酶
Gold theragran salvia miltion＇hiza prescription（GTSMP）
isehemia reperfusion（I/R）, protein kinase C （PKC）
iNOS
