摘要
目的:建立金丝桃苷的HPLC含量测定方法,控制山楂调中丸的质量。方法:采用高效液相色谱法,色谱条件Hy-persilODSC18柱(250×4.6mm,5μm);流动相:乙腈-甲醇-四氢呋喃-0.5%冰醋酸(1:1:19.6:78.4);流速:0.8mL/min;检测波长:363nm;进样量:供试品5μL,对照品20μL。采用外标法计算样品中金丝桃苷的含量。结果:金丝桃苷的线性关系范围10.84～97.56μg;回归方程:Y=31839X-13664,R=0.9998,平均回收率:98.202%,RSD为2.61%(n=6)。三批山楂调中丸中金丝桃苷的含量测定结果分别为50.3783μg、53.5741μg、48.0312μg。结论:该方法制备简便、分离效果好,处方中其他成分无干扰,提示该成分在该条件下具有检测专属性和唯一性,该方法可作为该药含量测定质控有效方法之一。
Objective： To establish hyperoside assaying process with HPLC for quality control of Pill Shanzha Tiaozhong （PST）. Method： HPLC was adopted. Chromatographic condition was Hypersil ODS ClS （250×4.6 mm, 5 μm）. Mobile phase was acetonitrile- methanol- tetrahydrofuran - 0.5 % glacial acetic acid （AN-MeOH-THF-0.5% glacial acetic acid） （1 ： 1 ： 19.6 ： 78.4）. Flow rate was 0.8 mL/min. Detecting wavelength was 363 nm. Sample size was test article 5 μL and control article 20 μL. Calculating Cont. hyperoside was with external reference method. Result： -The linear range of hyperoside was 10.84-97.56 μg, regression equation was Y=31 839X-13 664,R=0. 999 8 . The results of hyperoside assaying in three batches of PST were 50. 378 3 μg, 53. 574 1 μgand 48. 031 2 μg. Conclusion： The process is reliable and convenient for preparation and precise for separation and non-interference of other compositions. It suggests that the ingredient is specific and unique in detection under this condition, and the process can be as one of effective methods of assaying hyperoside for quality control of PST.
出处
《山西中医》
2007年第5期66-67,共2页
Shanxi Journal of Traditional Chinese Medicine
基金
山西省科学技术发展计划项目
编号2006031079
