摘要
目的测定不同产地黄蜀葵花药材中金丝桃苷,异槲皮苷,槲皮素-3'-葡萄糖苷含量。方法高效液相色谱法。色谱柱:Diamonsil C18柱(150mm×4.6mm,5μm);流动相:乙腈-甲醇(10∶1)-0.4%磷酸,梯度洗脱;检测波长:360nm;柱温:室温;流速:1.0ml/min。结果江苏江堰黄蜀葵花中金丝桃苷含量1.12%,RSD为1.54%;异槲皮苷含量0.68%,RSD为1.58%;槲皮素-3'-葡萄糖苷含量0.59%,RSD为1.47%;江苏兴化黄蜀葵花中金丝桃苷含量0.98%,RSD为1.49%;异槲皮苷含量0.60%,RSD为1.62%;槲皮素-3'-葡萄糖苷含量0.52%,RSD为1.55%;河北邢台黄蜀葵花中金丝桃苷含量1.03%,RSD为1.72%;异槲皮苷含量0.62%,RSD为1.84%;槲皮素-3'-葡萄糖苷含量0.54%,RSD为1.62%。结论江苏江堰黄蜀葵花中金丝桃苷,异槲皮苷,槲皮素-3'-葡萄糖苷含量均最高,江苏兴化黄蜀葵花中金丝桃苷,异槲皮苷,槲皮素-3'-葡萄糖苷含量均最低,该方法简便,准确,重现性好,为各地黄蜀葵花药材的进一步开发利用提供质量控制依据。
Aim To determine the contents of Hyperoside, Isoquerditrin and Quercitrin-3 ＇-glucoside in Flos Abelmoschus manihot of different areas. Methods The column was Diamonsil ClS （ 150mm × 4.6mm, 5μm）. The mobile phase was methanol - acetonitrile（ 10：1 ） - 0.4% phosphoric acid. The flow was 1.0ml/min. The detection wavelength was 360nm. The column temperature was room temperature. Results The content of Hyperoside in the drug of Jiangyan is 1.12% with RSD 1.54% .The content of Isoquerditrin in the drug of Jiangyan is 0.68% with RSD 1.58% .The content of quercitrin-3＇-glucoside in the drug of Jiangyan is 0.59% with RSD 1.47% .The content of Hyperoside in the drug of Xinghua is 0.98% with RSD 1.49% .The content of Isoquerditrin in the drug of Xinghua is 0.60% with RSD 1.62%. The content of Quercitrin-3＇-glucoside in the drug of Xinghua is 0.52% with RSD 1.55% .The content of Hyperoside in the drug of Xingtai is 1.03 % with RSD 1.72 %. The content of Isoquerditrin in the drug of Xingtai is 0.62 % with RSD 1.84%. The content of Quercitrin-3＇-glucoside in the drug of Xingtai is 0.54% with RSD 1.62%. Conclusion The contents of Hyperoside, Isoquerditrin and Quercitrin-3＇-glucoside in the drug of Jiangyan are all the highest, the contents of Hyperoside, Isoquerditrin and Quercitrin-3＇-glucoside in the drug of Xinghua are all the lowest. This method is simple, accurate and available for the exploitation and the quality control of the drug in different areas.
出处
《解放军药学学报》
CAS
2005年第5期388-390,393,共4页
Pharmaceutical Journal of Chinese People's Liberation Army
