摘要
目的观察喘复康合剂时支气管哮喘大鼠气道重塑的影响及其可能的作用机制。方法采用卵白蛋白致敏法复制大鼠哮喘模型,以喘复康合剂灌胃给药后,用免疫组织化学法检测各实验组大鼠肺组织中CD_(44)表达的变化;通过图像分析系统测定各实验组大鼠支气管壁各层的厚度;通过Masson三色染色法及图像分析系统测定各实验组大鼠胶原沉积厚度。结果哮喘组肺组织中CD_(44)的含量明显高于对照组(P<0.05),4周组较3周组更为明显;激素组CD_(44)表达不明显,与对照组相比无显著性差异(P>0.05)。喘复康低、高剂量组CD_(44)的含量明显低于哮喘组(P<0.05)。图像分析结果显示,哮喘组支气管平滑肌明显增厚,胶原沉积增加,4周组较3周组更为明显,与对照组比较有显著性差异(P<0.05),激素组与喘复康组平滑肌增厚不明显,与对照组相比无显著性差异(P>0.05)。结论喘复康合剂能够抑制哮喘大鼠气道重塑的形成,可能是通过减少肺组织中CD_(44)的产生和表达而发挥的,与激素作用结果相似。
Objective Try to observe the effect and possible mechanism of ChuanFuKang mixture on airway remodell of rat models of asthma. Methods An animal mQdel of asthma was established by OVA （ovalbumin） sensitizing - challenging SD rat, Water extract of ChuanFuKang were given to rats（ig）. Immunohistochemical technology was used to estimate the change of CD44 in the rat lung tissues of each group, the thickness of bronchial wall and the area of collagen deposition was detected by computer image analyse system and by method of Masson trichrome staining. Results The content of CD44 of lung tissue of asthma group was significantly higher than that of control group （P 〈 0.05）, the four - week group was more obvious than the three - week group. The content of CD., of low dose and high dose group was obviously lower than that of asthma group（ P 〈 0.05）. The results of the image analysis showed that the thickness of bronchial smooth muscle and the area of collagen deposition obviously increased, the four -week group was more evident than the three -week group, there was significant difference compared with control group （P 〈 0.05）. These changes of hormone group and ChuanFuKang group were not evident, there was no significant difference compared with control group（ P 〉 0.05）. Conclusion ChuanFuKang mixture can inhibite the airway remodeling by reducing the produce and the expression of CD44 in asthmatic rat lung tissue.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第9期2192-2194,共3页
Lishizhen Medicine and Materia Medica Research
关键词
哮喘
气道重塑
喘复康大鼠
Asthma
Airway remodeling
ChuanFuKang Rats
