摘要
据胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae,APP)生物I型各血清型之间外毒素(apx)基因组结构差异,参考GenBank上已发表的4种毒素(apxI/apxII/apxIII/apxIV)的核酸序列设计了6对特异性引物,对APP的12个血清型进行多重PCR扩增,得出不同的特异性的扩增片段,得以准确鉴定APP生物I型中的9种血清型,但其中血清型2和8,血清型5、9和11仍未能区分。
Actinobacillus pleuroneumoniae（ A. pleuropneumoniae） is an important swine respiratory disease pathogen, which has at least 15 semtypes. There are several techniques for the serotyping of A. pleuropneumoniae, however, these techniques are time consuming. In this study, the polymerase chain reaction （PCR）technique was developed for semtyping A. pleuropneumoniae using a set of specific primer designated for the apx Ⅰ ,apx Ⅱ ,apx Ⅲ and apxⅣ genes. By this PCR typing system,9 out of the 12 reference strains of A. pleuropneumoniae were differentiated. However, it was unable to distinguish serotype 2 from 8, semtype 5 and semtype 9 form 11. The PCR typing system could be further applied for typing the field isolates of A. pleuropneumoniae.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2007年第5期653-656,共4页
Chinese Journal of Veterinary Science
基金
上海市科委科研基金资助项目(027205026)
关键词
胸膜肺炎放线杆菌
血清型
PCR
apx基因
Actinobacillus pleuropneumoniae
semtype
polymerase chain reaction
apx gene
