摘要
APXⅣA基因是新发现的猪传染性胸膜肺炎放线杆菌(APP)的RTX毒素基因,本文通过PCR方法从APP标准血清1型及临床分离到的3株野毒株中扩增出长为442bp的部分APXⅣA基因,而从其它引起猪呼吸道疾病的细菌中无法扩增出此片段。通过玻板凝集试验鉴定这三株野毒株为血清1型APP。所建立的PCR方法能检测的DNA量最高敏感度为28pg.表明本文所建立的对猪传染性胸膜肺炎放线杆菌的PCR诊断方法具有较高的特异性和敏感性。
The APXⅣA gene is a recently discovered RTX determinant of Actinobacillus pleuropneumoniae.A primer pair spanning this region specifically amplified a 442 bp fragment in PCR experiments with DNA from serotype 1 strain and three field strains. These field strains were identified as serotype 1 by agglutination.The PCR did not amplify any product when DNA from other bacterial species closely related to swine respiratory diseases was used as template. The sensitivity of the PCR was determined to be 28 pg of Actionbacillus pleuropneumoniae DNA.The method of diagnosing actionbacillus pleuropneumoniae was show to be specific and sensitive.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2004年第1期36-38,共3页
Chinese Journal of Preventive Veterinary Medicine
